摘要
利用火焰原子吸收法对家兔脑缺血再灌注损伤后血钙的含量进行检测,并与假手术组进行比较,结果显示缺血再灌注损伤组血液中的钙含量高于假手术组(P<0.01),差异有统计学意义,并且缺血再灌注2h组和缺血再灌注4h组中差异也有明显统计学意义(P<0.05),本实验提示家兔发生局部脑缺血再灌注损伤后血液中的钙离子较正常状态下升高,并可通过火焰原子吸收法有效地测定血液中钙的含量。
We determined the blood calcium of rabbits with cerebral ischemia-reperfusion injury by flame atomic absorption spectrophotometry,and compared with sham operated controls, the results indicated that the blood calcium of rabbits with cerebral ischemia-reperfusion injury decreased obviously(P〈0.01). It have disparity between the two of cerebral ischemia-reperfusion injury groups(P〈0.05). Our experiments displayed that the blood calcium of rabbits with cerebral ischemia-reperfusion injury decreased, and we could make use of flame atomic absorption speetrophotometry method to determine calcium content in blood.
出处
《成都医学院学报》
CAS
2012年第4期573-575,共3页
Journal of Chengdu Medical College
基金
成都医学院大学生开放实验项目基金(NO:KF2011)
关键词
缺血再灌注损伤
原子吸收法
血钙
cerebral ischemia reperfusion injury
atomic absorption
blood calcium