摘要
目的:观察不同剂量的解毒通络方对脑缺血损伤后大鼠学习记忆能力的影响。方法:实验于2005-06/07在北京中医药大学病理实验室和中国医学科学院药用植物研究所神经药理试验室完成。选择健康清洁级SD大鼠120只,按随机数字表法选择20只作为正常对照组,其余大鼠采用反复夹闭双侧颈总动脉结合硝普钠降压法复制SD大鼠拟血管性痴呆模型。造模后的大鼠随机分为模型组、脑心通组和解毒通络方1.85g/kg,3.7g/kg和7.4g/kg组,每组各20只。脑心通组和解毒通络方1.85g/kg,3.7g/kg和7.4g/kg组分别灌胃给予0.084g/mL的脑心通胶囊混悬液、1.85g/kg,3.7g/kg和7.4g/kg的解毒通络方(由栀子、黄芪、丹参等组成)稀释液5.0mL/kg。正常组与模型组同样按照5.0mL/kg的体积每日用纯净水灌胃。连续给药4周后进行Morris水迷宫测试,通过变换平台位置对大鼠空间定向能力(探索阶段)及再学习能力(再学习阶段)进行评价,比较潜伏期和平均速度。结果:进入结果分析6组大鼠共92只。①在探索阶段,模型组的潜伏期时间显著长于正常对照组(P<0.01),解毒通络方3.7g/kg和7.4g/kg组与模型组比较潜伏期显著缩短(P<0.01);模型组与正常对照组比较平均速度显著降低(P<0.01),解毒通络方1.85g/kg组与模型组比较平均速度显著提高(P<0.05)。②在再学习阶段,模型组潜伏期与正常对照组比较显著延长(P<0.01),解毒通络方3.7g/kg和7.4g/kg组则较模型组显著缩短(P<0.05);模型组平均速度显著低于正常组(P<0.01),同时解毒通络方3个剂量组与模型组比较平均速度均有显著提高(P<0.05)。结论:解毒通络方3.7g/kg,7.4g/kg组的疗效比较明显,在大鼠寻找平台平均潜伏期的缩短和平均速度的提高上都表现出显著的药物治疗作用;而1.85g/kg组的疗效则主要体现在提高平均速度上。提示解毒通络方能够明显改善血管性痴呆大鼠的空间探索与定向能力,提高其学习与再学习能力。
AIM： To observe the effects of Jiedu Tongluo Fang at different dosages on learning and memory abilities in rats with cerebral ischemic injury.
METHODS： The experiment was conducted from June to July 2005 at the Pathological Laboratory, Beijing University of Traditional Chinese Medicine and the Neuropharmacological Laboratory, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences. Totally 120 healthy SD rats of cleaning grade were randomized into control group （n=20） and vascular dementia models, which were duplicated by repeated clamping of bilateral common carotid artery combined with sodium nitroprusside depressing. Then the models were divided into 5 groups： model group, Naoxintong group and experimental groups including high dose, medium dose and low dose Jiedu Tongluo Fang, with 20 rats in each. 0.084.g/mL Naoxintong capsule suspension and 5.0mL/kg Jiedu Tongluo Fang diluted solution （consisted of cape jasmine fruit, membranous milkvetch root and dan-shen root） at different dosages were given intragastrically in the Corresponding groups. And intragastric infusion of 5.0 mL/kg purified water was administered daily into the rats of control group and model group. Four weeks of continuous infusion later, Morris water maze was applied to test the spatial orientation （searching stage） and relearning （relearning stage） abilities by changing the platform position, and the escape latency and average speed were compared.
RESULTS： Totally 92 rats in 6 groups were involved in the result analysis. ①During the searching stage, compared with control group, the search latency prolonged markedly but the average speed reduced markedly in model group （P 〈 0.01）. Compared with model group, the search latency of Jiedu Tongluo Fang groups of high dose and medium dose were much shortened （P 〈 0.01）, while the average speed notably increased in the low dose group （P 〈 0.05）.②At the relearning stage, the latencies were significantly longer in models than in controls （P 〈 0.01）, while much shorter in Jiedu Tongluo Fang groups of high dose and medium dose compared with models （P 〈 0.05）. The average speed was significantly lower in model group than in control group （P 〈 0.01）, and three experimental groups all had higher speed compared with model group （P 〈 0.05）. CONCLUSION： Jiedu Tongluo Fang of medium dose and high dose are obviously effective in shortening the search latency and elevating the average speed of rats, whereas the low dose only elevates the average speed. It is indicated that Jiedu Tongluo Fang can remarkably facilitate the spatial searching and orientation, and rise the learning and memory abilities in rats with vascular dementia.
出处
《中国临床康复》
CSCD
北大核心
2006年第23期36-39,共4页
Chinese Journal of Clinical Rehabilitation
基金
国家重点基础研究发展规划课题
通络方药对缺血性损伤脑组织保护作用的微血管机制研究(2005CB523311)~~
