摘要
以玉米芯纤维素为基质,通过环氧氯丙烷(EPI)交联活化、亚氨基二乙酸(IDA)修饰、金属离子Cu,Fe,Zn,Ni螯合制得亲和吸附剂.通过红外光谱(FTIR)、X射线光电子能谱(XPS)、原子吸收分光光度法(AAS)对其表征.考察了pH、离子强度、初始浓度、洗脱液等因素对螯合了不同金属离子的吸附剂吸附牛血清白蛋白(BSA)的影响.结果表明:对强亲和性的Cu(Ⅱ)螯合亲和吸附剂对BSA的吸附主要受配位作用控制,而对弱亲和性的Fe(Ⅱ)、Zn(Ⅱ)、Ni(Ⅱ)螯合亲和吸附剂则主要受静电作用影响,配位作用为辅.
Several biosorbents were successfully prepared using coin cellulose as supporting materials, epichlorohydrin （EPI） as crosslinking agent and iminodiacetic acid （IDA） as modification agent. Adsorbents with metal-chelated affinity were obtained by chelating with Cu, Fe, Zn and Ni metal ions respectively. These novel adsorbents were characterized with FIIR, XPS and AAS. The effects of pH, ionic strength, initial concentration, desorbents on the adsorption of adsorbents for BSA were studied. It was proposed that the retention of protein on Cu （ 11 ） chelating affinity adsorbent was mainly dominated by the coordination role between the immobilized metal and protein. The protein retention on Fe（ II ）, Zn（ lI ） and Ni（ 11 ） chelating affinity adsorbents with weak affinity was mainly controlled by the electrostatic interaction between metal chelating ligand and protein, whereas the coordination role was additional in the protein retention.
出处
《中南民族大学学报（自然科学版）》
CAS
2012年第4期1-5,共5页
Journal of South-Central University for Nationalities：Natural Science Edition
基金
国家民委院校科研基金资助项目(MJY00004)
