摘要
采用反相悬浮包埋技术制备了粒径小于３００μｍ、粒径分布窄和湿态孔度高（８５％～９０％）的高顺磁性珠状纤维素．经高碘酸钠活化后，与具有生物活性的绒毛膜促性腺激素偶联，得磁性亲和吸附剂（每克磁性珠状纤维素上固载３００～４００ＩＵ绒毛膜促性腺激素）．
In this paper,the reversed phase suspension and embedment techniques were adopted of preparing the paramagnetic cellulose beads (MCBs). While the viscose suspended in oil phase was heated at 90 ℃ for 40 min, cellulose might be regenerated from cellulose xanthate,and the γ Fe 2O 3 superfine powder(<300 nm) dispersed in the viscose was wrapped up among the regenerated cellulose chains, then the MCBs were obtained whose mean size might be less than 300 μm , and its distribution was narrow.Moist MCBs have the porosity more than 85%. Their magnetizability was of the order of magnitude of 10 -3 emu/g. These MCBs can be activated by many kinds of common methods, and can be applied to immobilizing different bioactive ligands to obtain various magnetic affinity adsorbents(MAA). We activated the MCBs in a 35 mmol/L sodium periodate solution, and immobilized the human chorionic gonadotropin(hCG) on them to obtain the MAA with 300 IU hCG/g content. This kind of MAA could specifically adsorb anti hCG antibody from murine ascites supernatant.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1998年第6期994-999,共6页
Chemical Journal of Chinese Universities
基金
天津市自然科学基金
关键词
纤维素
促性腺激素
亲和吸附剂
绒毛膜
MCB
MAA
Cellulose, Magnetic resin, Affinity adsorbent, Human chorionic gonadotropin (hCG)
