摘要
目的观察芡实80%乙醇、乙酸乙酯、正丁醇提取物对过氧化氢(H2O2)诱导的SH-SY5Y神经细胞损伤的保护作用及其体外抗氧化活性。方法采用体外细胞培养的方法,建立SH-SY5Y神经细胞H2O2损伤模型。用Cell Counting Kit-8(CCK-8)试剂盒法检测以上不同浓度芡实提取物对H2O2诱导损伤的SH-SY5Y神经细胞活力的影响,计算抑制率。用体外抗氧化实验方法检测芡实3种提取物清除DPPH、ABTS自由基及还原铁的能力。结果芡实80%乙醇、乙酸乙酯、正丁醇提取物在含生药量10～30mg/mL浓度范围内,均表现出明显的抑制H2O2对SH-SY5Y神经细胞氧化损伤的作用(P<0.05,P<0.01),且对DPPH、ABTS自由基及三价铁离子有很好的清除作用及还原能力,其中芡实正丁醇提取物的作用最强。结论芡实具有保护SH-SY5Y神经细胞免受H2O2氧化损伤的作用,有着很好的抗氧化活性,其中正丁醇提取物的抗氧化活性最强。
OBJECTIVE To investigate the protective effects of euryale seed 80% ethanol, ethyl acetate and n-butyl alcohol extracts on the H2O2-induced damaged SH-SY5Y neurocytes and their invitro antioxidant activities. METHODS The H2O2- induced damaged SH-SY5Y neuroeyte models were cultured outside of the body. Cell Counting Kit-8 （CCK-8） was used to de- termine the effects of the above euryale seed extracts with different concentrations on the activities of the H2O2-induced dam- aged SH-SY5Y neurocytes, and then their inhibition rates were calculated. The in-vitro antioxidant experimental method was applied to detecting the capabilities of the three extracts in removing DPPH, ABTS radicals and deoxidating three price iron i- ons. RESULTS Within the crude drug concentration range of 10~30mg/mL, euryale seed extracts in 80% ethanol, ethyl ace- tate and n-butyl alcohol showed obvious functions of inhibiting the oxidant damage action of H2O2 on the SH-SY5Y neuroeytes （P〈0.05, P〈0.01）. In addition, the above euryale seed extracts could markedly clear away DPPH, ABTS radicals and deox- idate three price iron ions, and among which, the n-butyl alcohol extract had the most powerful action. CONCLUSION Having an excellent antioxidant activity, euryale seed can protect the SH-SY5Y neurocytes from the damage of H2 O2. Its active ex- tracting solvents include ethanol, ethyl acetate and n-butyl alcohol. After a comprehensive comparison, it shows that the n-bu- tylalcohol extract has the most powerful function.
出处
《南京中医药大学学报》
CAS
CSCD
北大核心
2013年第1期39-43,共5页
Journal of Nanjing University of Traditional Chinese Medicine
基金
国家"十二五"支撑计划项目(2011BAI04B06)
江苏省高校中药资源与化学研究科技创新团队(2011)
江苏省六大人才高峰项目(2009)
