摘要
目的 :运用中药血清药理学方法研究补肾复方含药血清 (BS)促进体外培养的PC12细胞增殖及其拮抗谷氨酸兴奋性神经毒损伤的作用。方法 :以MTT法观察BS对体外培养的PC12细胞活力的影响及其拮抗谷氨酸损伤的作用 ,采用流式细胞术观察BS对谷氨酸所诱导PC12细胞凋亡的影响。结果 :BS含药血清可以增强PC12细胞活力 ,拮抗谷氨酸的兴奋性神经毒作用且在 2 0 %浓度时作用最强 ,并且能够抑制谷氨酸诱导的PC12细胞早期凋亡。结论 :补肾方BS可能具有神经保护作用。
Objective:The enhancing effect of serum with Bu Shen Decoction (BS) on cultured PC12 cells proliferation and its antagonistic action on neurotoxicity induced by glutamate (Glu) were investigated with serum pharmacology method of the traditional Chinese medicine in vitro. Methods:the effect of BS on cultured PC12 cells activity and its antagonistic action on neurotoxicity induced by Glu was observed with MTT method.Flow Cytometry and Fluorescence microscope techniques was employed to observe the effect of BS on PC12 cells early period apoptosis induced by Glu.Results:The serum with BS was discovered to be able to reinforce PC12 cells activity and exert antagonistic effect on Glu induced neurotoxicity.Meanwhile,These beneficial effects afforded by BS were found to be the strongest on either enhance proliferation of PC12 cells or antagonize neurotoxicity induced by glutamate in 20% concentration of serum with BS.Moreover,it can inhibit apoptosis of PC12 cells induced by Glu which occurred in the early period.Conclusion:BS might exert a potential neuroprotective effect.
出处
《中国中医基础医学杂志》
CAS
CSCD
北大核心
2005年第3期184-188,共5页
JOURNAL OF BASIC CHINESE MEDICINE
基金
山西省自然科学基金资助项目 (199910 99)
关键词
补肾复方
神经保护
细胞凋亡
中药血清药理学
PC12细胞
Bu Shen Decoction
Nerve protection
apoptosis
serum pharmacology method of the traditional Chinese medicine
PC12 cells
