摘要
应用血清中和试验 (SNT)和伪狂犬病乳胶凝集试验 (LAT)诊断试剂盒对两种伪狂犬病标准阳性血清、伪狂犬病病毒 (PRV)高免血清及 6 0份被检猪血清进行了PRV抗体效价测定和相关性分析 ,两种方法测得的抗体效价之间呈强相关性 (r=0 .96 ) ,且LAT效价比SNT一般高出一个滴度 ;对来自 35个猪场的 414份猪血清进行了PRV抗体检测 ,并与SNT检测结果进行了对比 ,结果在SNT检测为阳性的 171份血清中 ,LAT检出阳性 16 8份 ,阳性符合率为 98.2 % (16 8/ 171) ,在SNT检测为阴性的 2 43份血清中 ,LAT检出 5 2份血清为PRV抗体阳性 ;对猪瘟、口蹄疫 (O型 )、猪细小病毒、猪衣原体四种标准阳性血清和来自无伪狂犬病猪场的 18头健康猪血清进行检测 ,检测结果均为阴性 ,其符合率为 10 0 % (2 2 / 2 2 )。结果表明 ,两种方法检测结果符合率高 ,特异性强 ,LAT比SNT敏感、快速简便和实用。
Two kinds of standard positive sera against PRV,a hyperimmune serum to PRV,and 60 sera from pig farms were titrated by LAT kit for diagnosing pseudorabies and SNT,and both methods correlativity was analyzed;it is strong correlativity between LAT and SNT titers(r=0.96),the LAT determined a titer higher than SNT generally,414 sera from 35 piggeries were tested the antibody against PRV by LAT and SNT,168 of 171 seropositive sera detected by SNT were positive to PRV by LAT,the positive coincidence rate of both methods reached 98.2%(168/171),52 of 243 seronegative sera determined by SNT were positive to PRV.Both methods were negative reaction with swine fever (SF)、o type foot_and _mouth disease (FMD)、porcine parvovirus (PPV)、chlamydia standard positive sera and 18 sera from a health herd known to be free of pseudorabies,the coincidence rate of both methods was up to 100%(22/22).The results indicated that the coincidence rate of both methods was very high,this LAT had strong specificity and was more sensitive and rapid and simple and practical than SNT.
出处
《中国预防兽医学报》
CAS
CSCD
2000年第4期302-305,共4页
Chinese Journal of Preventive Veterinary Medicine
关键词
猪伪狂犬病
抗体检测
SNT
LAT
Pseudorabies
Latex agglutination test (LAT)
Serum neutralization test (SNT)
Antibody detection
