摘要
目的探讨精制大黄虫丸抗动脉血栓形成作用及机制,观察钛铝金属间化合物多孔材料对药物作用的影响。方法 50只SD大鼠随机分为正常组、模型组、大黄虫丸组、精制方组、多孔材料组,每组各10只。除正常组外,其余大鼠通过三氯化铁诱导动脉血栓形成模型,大黄虫丸组给予大黄虫丸,精制方组大鼠给予精制大黄虫丸,多孔材料组大鼠给予通过钛铝金属间化合物多孔材料处理的精制大黄虫丸方药,各给药组根据体重按1ml/100g灌胃,每天2次,共1周;模型组、正常组给予等体积生理盐水灌胃。光学显微镜下观察大鼠颈动脉血管组织病理变化,记录各组大鼠血栓干重,检测血小板计数、血液流变学、活化部分凝血酶时间(APTT)、凝血酶原时间(PT)及血栓素B2(TXB2)和6-酮-前列腺素F1α(6-Keto-PGF1α)的水平。结果与模型组比较,大黄虫丸组、精制方组和多孔材料组血栓干重减轻(P<0.05);多孔材料组较精制方组和大黄虫丸组血栓干重降低明显(P<0.05)。与正常组相比,模型组血小板计数、TXB2水平明显增加,6-keto-PGF1α降低(P<0.05);与模型组比较,大黄虫丸组、精制方组和多孔材料组均可以明显降低血小板计数和TXB2水平,升高6-keto-PGF1α(P<0.05);多孔材料组较精制方组和大黄虫丸组降低血小板计数和TXB2水平明显(P<0.05)。与正常组比较,模型组血液黏度增高、APTT和PTT缩短(P<0.05);与模型组比较,大黄虫丸组、精制方组和多孔材料组血液黏度降低,APTT和PTT均明显延长(P<0.05)。结论精制大黄虫丸能明显抑制大鼠动脉血栓形成,能改善血液流变性,明显延长APTT和PTT,其机制可能与升高6-keto-PGF1α的水平、降低TXB2的水平有关。钛铝金属间化合物多孔材料可应用于中药的分离筛选以提高疗效。
Objective To research the effect and mechanism of Refined Dahuang Zhechong Pill [Refined Rhubarb （Radix et Rhizoma Rhei） and Ground Beetle （Eupolyphaga seu Steleophaga） Pill, RDZP] on arterial thrombosis and observe the influence of titanium-aluminum intermetallic compound porous material on drug action. Methods Fifty SD rats were randomized into the normal group, model group, DZP group, RDZP group and porous material group, with 10 in each. The arterial thrombosis models were induced by ferric chloride in all groups except normal group. DZP, RDZP and RDZP processed by titanium aluminum intermetallic compound porous material were given to the DZP group, RDZP group and porous material group respectively. Each administration group was given 1ml/100g of corresponding drug solution intragastrically based on body weight twice daily for one week. The normal group and model group were given the same volume of saline intragastrically. The pathological situation of carotid artery in rats was observed by microscope. The dry weight of thrombosis, platelet count, hemorheology, activated partial thromboplastin time （APTT）, prothrombin time （PT） blood coagulation and the content of thromboxane B2 （TXB2） and 6 keto prostaglandin F1a（6-Keto-PGF1a） were recorded. Results The thrombosis could be seen in all modeling groups and the dry weight of thrombosis in the model group was the heaviest. Comparing with the model group, the dry weights of thrombosis were significantly reduced in the DZP group, RDZP group and porous material group （P〈0.05）, especially in the porous material group （P〈0.05 or P〈0.01）. Comparing with the normal group, the platelet count and TXB2 content were significantly increased and 6-keto-PGF1a content were significantly decreased in the model group （P〈 0.05）. Comparing with the model group, the platelet count and TXB2 content were significantly decreased and 6-keto-PGF1a, content were significantly increased in the DZP group, RDZP group and porous material group （P〈0.05）. Comparing with the DZP group and RDZP group, the platelet count and TXB2 content were significantly decreased in the porous material group （P〈0.05 or P〈0.01）. Comparing with the normal group, the blood viscosity was significantly increased but APTTand PT were significantly shortened in the model group （P〈0.05）. Comparing with the model group, the blood viscosity was significantly decreased but APTTand PT were significantly prolonged in the DZP group, RDZP group and porous material group （P〈 0.05）. Conclusion RDZP can obviously inhibit arterial thrombosis, improve blood rheology and prolong APTTand PT that may be relevant to increasing 6-keto-PGFlo content and decreasing TXB2 content. Titanium-aluminum intermetallic compound porous material can be applied in the separation and filtering of Chinese medicinal to improve efficacy.
出处
《中医杂志》
CSCD
北大核心
2013年第2期138-141,共4页
Journal of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(81173198)
2010年度国际科技合作专项资助项目(2010DFA32370)
"十一五"国家科技支撑计划(2009BAI80B01)
湖南省科学技术厅科技计划重点项目(2008WK3002)
中国博士后科学基金资助项目(20060390891)
关键词
钛铝金属间化合物多孔材料
精制大黄蟅虫丸
动脉血栓
titanium-aluminum intermetallic compound porous materials Refined Dahuang Zhechong Pill
arterial thrombosis
