摘要
目的:建立黑豆皮中总黄酮的含量测定方法,测定八个不同产地黑豆皮中总黄酮的含量。方法:以芦丁为对照品,采用硝酸铝显色,比色法在510nm处测定不同产地的黑豆皮中总黄酮的含量,并考察了黑豆皮中总黄酮的最佳处理方法,保证测定结果的准确性。结果:芦丁在0.003～0.057mg/mL范围内吸光度与浓度呈良好的线性关系,标准曲线回归方程为:Y=12X-0.0009(R2=0.9999),平均回收率为104.6%。不同产地黑豆皮中总黄酮的含量在6.873%～17.017%之间。结论:所用方法简便、准确,结果显示:不同产地的黑豆皮中总黄酮含量差异较大,山西和安徽的四种黑豆皮中含量较高。
Objective：To determine the flavonoids content in black soybean seed coat from eight different regions by colormetric method.Methods：Colormetric method was used to determine the total flavonoid contents in black soybeen seed coat from diferent regions at wavelength 510nm by taking rutin as standard substance,and Al（NO4）3 as chromogenic reagent.The optimum extraction process of total flavonoids in black soybean seed coat was selected for ensure accuracy of the results.Results：The linear equation was Y=12X-0.0009（R2=0.9999）,and the calibration curve was linear in the range of 0.003 ~ 0.057mg/mL.The average recovery was 104.6%.The contents of total flavonoids in black soybean seed coat from different regions were 6.873% ~ 17.017%.Conclusion：The method was simple and rapid.The result showed that different origin of the content was quite different,with higher levels of Shanxi and Anhui.
出处
《食品工业科技》
CAS
CSCD
北大核心
2013年第1期309-311,316,共4页
Science and Technology of Food Industry
基金
天津市科技支撑计划资助(09ZCKFNC01000)
关键词
比色法
黑豆皮
总黄酮
含量测定
Colorimetric method
black soybean seed coat
total flavonoids
the determination of content
