摘要
研究了搅拌转速、pH控制以及结合摇瓶发酵过程中不同时间硫酸铵的补加对 β 1,4 聚糖酶形成的影响 ,优化得到A 30的 β 1,4 聚糖酶分批发酵操作条件和初步优化了 (NH4 ) 2 SO4 流加发酵条件。研究结果显示麸皮表面有大量A 30菌体细胞的吸附 ,搅拌转速对菌体吸附和 β 1,4 聚糖酶的形成有明显影响 ;发酵过程中 pH下降有利于 β 1,4 聚糖酶形成。采用了初期以 5mL/h的速率恒速流加 ,后期测定 (NH4 ) 2 SO4 浓度进行调整的流加方法 ,使木聚糖酶活达到 6 16IU/mL ,纤维素酶活达到 1 33UI/mL。
The effects of stirring rate,pH control and feed time of (NH 4) 2SO 4 on β 1,4 glycanases were investigated.The operating conditions of batch fermentation and fed\|batch culture were optimized.The results showed there were a large amount of bacterial cells adsorbing on the surface of wheat bran,and stirring rate had a pronounced influence on the adsorption and the production of β 1,4 glycanases.The uncontrolled pH could promote the induction of β 1,4 glycanases.When (NH 4) 2SO 4 was fed at a constant rate in the earlier age,and regulated later by determining the content of (NH 4) 2SO 4,xylanase and CMCase activity could reach 616IU/mL and 1 33IU/mL respectively.
出处
《生物工程学报》
CAS
CSCD
北大核心
2000年第4期485-489,共5页
Chinese Journal of Biotechnology
基金
国家"九五"攻关项目!(96C0 3 0 2 0 3 )
