摘要
【目的】筛选能够适应南方酸性土壤的高效半纤维素降解菌株,并进行鉴定,确定菌株的安全性。【方法】采用半纤维素平板水解圈法和胞外酶测定法进行菌株筛选,通过拮抗实验构建复合微生物菌系。利用培养特征、形态学、生理生化特征和分子生物学方法进行菌株鉴定。【结果】筛选出效果稳定,互不拮抗的高效半纤维素降解放线菌4株(NA9、NA10、NA12和NA13),半纤维素酶活分别为:217.6、229.8、221.1和211.8 U/mL。真菌2株NF1和NF7,半纤维素酶活为217.7和244.2 U/mL。复合微生物菌系半纤维素酶活可达299.0 U/mL。经鉴定菌株NA9、NA10、NA12和NA13为链霉菌中的哥斯达黎加链霉菌(Streptomyces costaricanus)。菌株NF1为亮白曲霉(Aspergillus candidus),菌株NF7为黄蓝状菌(Tarlaromyces flavus)。
[Objective] The aim of this study was to screen hemicellulose degrading microorganisms.[Methods] The methods used to screen the effective strains included hydrolysis spot diameter measurement of hemicellulose plate and extracellular enzyme activity.The methods used to identify the strains included culture characteristics,morphological,physiological-biochemical characteristics and molecular biological methods.[Results] We isolated 4 actinomycetes（NA9,NA10,NA12 and NA13）,2 fungi（NF1 and NF7） with hemicellulose degrading ability and no antagonistic effect among them.The hemicellulose degrading activity of 4 actinomyces（NA9,NA10,NA12 and NA13） was 217.6,229.8,221.1 and 211.8 U/mL.The hemicellulose degrading activity of 2 fungi（NF1 and NF7） was 217.7 and 244.2 U/mL.The hemicellulose degrading activity of complex microbial system was 299.0 U/mL.NA9,NA10,NA12 and NA13 were Streptomyces costaricanus;NF1 was Aspergillus candidus and NF7 was Tarlaromyces flavus.[Conclusion]the 4 actinomyces and 2 fungi screened have high hemicelluloses enzyme activity.These strains have good application value and more research value.
出处
《微生物学报》
CAS
CSCD
北大核心
2012年第10期1251-1259,共9页
Acta Microbiologica Sinica
基金
广东省教育部产学研结合项目(2011B090400277)
公益性行业(农业)科研专项经费(201003016)~~
关键词
酸性土壤
半纤维素降解菌
筛选
鉴定
acid soil； hemicellulose degrading microorganisms； screen； identification
