摘要
对河蟹眼柄神经分泌细胞的细胞学进行了研究，建立了河蟹眼柄视神经节终髓Ｘ器官 （ＭＴＸＯ）细胞原代培养的实用方法。ＭＴＸＯ神经分泌细胞在添加了谷氨酰胺和常量抗菌素的 Ｌ－１５培养基中表现出快速的再生生长，细胞生长可保持３—５ｄ，维持存活约１８ｄ。在ｐＨ６．８－ ７．８、温度 ２０℃－２８℃及渗透压 ９５０－１１００ｍＯｓｍ条件下，均能存活和生长，但在无 Ｃａ２＋或添 加 Ｃａ２＋通道阻断剂 ＣｄＣｌ２的培养基中不能生长。依据大小、形态、分布和超微结构特征及体外 生长方式区分出Ａ、Ｂ、Ｃ三种不同类型细胞。
the neurosecretory cells were studied in terms of cytology and primary culture in the MTXO of Eriocheir sinensis, and a practical method for primary culture of peptidergic neurons was set up. The peptidergic neurons, when dissociated from the MTXO, exhibited immediate outgrowth for 3-5 days and survived for 18 days or more in the defined medium supplemented with glutamine and antibiotics. The neurons could survive in some conditions involving changes of pH(7.0- 7.9). temperature(22℃- 28℃) and osmolarity (950- 1100mOsm). The outgrowth of the peptidergic neurons could be restrained in the Ca-free medium and blocked by Cd2+ (Cd2+ current blocker)in the medium. Three types of neurosecretory cells were distinguished on the basis of size, morphology, distribution, form of outgrowth and ultrastructure. There is an ultrastructural evidence that B, C types of neurosecretory cells have a rest phase in the development of Eriocheir sinensis.
出处
《水生生物学报》
CAS
CSCD
北大核心
2000年第4期374-379,407-408,共6页
Acta Hydrobiologica Sinica
关键词
河蟹
MTXO
神经内分泌
超微结构
细胞培养
Crustacea
Eriocheir sinensis
X-organ
Neurosecretory cells
Ultrastructure
Cell culture
