摘要
目的:观察自然周期排卵、促排卵与控制性超促排卵(COH)状态下小鼠卵巢生长分化因子-9(growth differentiation factor,GDF-9)表达水平的变化情况。方法:成年雌性小鼠随机分为3组每组10只,分别为自然排卵组(对照组)、孕马血清(PMSG)促排卵组(PMSG组)、控制性超促排卵组(COH组),各组小鼠取排卵期卵巢组织,采用免疫组织化学方法检测GDF-9的表达与定位,并分别采用Western blottting和实时荧光定量PCR法,定量检测GDF-9蛋白与mRNA的含量。结果:各组小鼠卵母细胞与颗粒细胞中均检测到GDF-9的表达,PMSG组与COH组GDF-9蛋白与mRNA的表达水平均显著高于对照组(P<0.05),PMSG组的表达也明显弱于COH组(P<0.05)。结论:促排卵可促进小鼠卵巢GDF-9的表达,但COH降低促排卵小鼠卵巢GDF-9的表达。
Objective： To investigate the expression of GDF-9 in mouse ovaries under natural ovulation, induced ovulation or controlled ovarian hyperstimulation （COH）, respectively. Methods： Adult female mice were divided into three groups： natural ovulation group （the control）, PMSG induced ovulation group （PMSG group） and controlled ovarian hyperstimulation group （COH group）, 10 mice per group. Ovary tissues were collected on ovulation period, and the expression and the location of GDF-9 were detected by immunohistochemistry method. The GDF- 9 protein was quantitated by Western blotting analysis and GDF-9 mRNA was quantitated by real-time fluorescence quantitative PCR. Results： The expression of GDF-9 was detected both in oocytes and granulosa cells by immunohistochemistry in all the three groups. In PMSG group and COH group, the expression of GDF-9 protein and mRNA levels were dramatically increased compared with the control （P〈0.05）. While the expression of GDF-9 was significantly down-regulated in COH mice compared with PMSG mice both in protein and mRNA levels （P〈0.05）. Conclusion： The expression of GDF-9 in mouse ovary was increased by induced ovulation, but the COH procedure deceased the GDF-9 levels compared with the induced ovulation.
出处
《生殖与避孕》
CAS
CSCD
2013年第1期1-5,共5页
Reproduction and Contraception
基金
广东省自然科学基金面上项目(10151001002000009)
国家自然科学基金面上项目(30973929)
