摘要
甘油 - 3磷酸转酰酶 (GPAT)与植物抗冷性密切相关。南瓜 (Cucurbitamoschata)与黑子南瓜 (Cucurbitaficifolia)同属不同种 ,亲缘关系较近 ,但却存在显著的抗冷性差异。南瓜及黑子南瓜GPAT基因的克隆 ,可以使我们从二者推导的有限氨基酸的差异中对GPAT氨基酸组成及其与植物抗冷性作一定的探讨。发现在南瓜与黑子南瓜 13个不同的氨基酸残基中有 3个与抗冷性植物拟南芥菜 (Arabidopsisthaliana)、豌豆 (Pisumsativum)、红花 (Carthamustincto rius)和菠菜 (Spinaciaoleracea)等相同 ,可能与黑子南瓜比南瓜更具抗冷性的原因有关。比较南瓜、黑子南瓜、豌豆、红花、拟南芥菜和菠菜等植物中GPAT基因推导的氨基酸序列发现 ,在比较抗冷的拟南芥菜、红花、豌豆和菠菜等植物中 ,虽然它们之间的亲缘关系都比较远 ,但某些位点上的氨基酸残基却完全相同 ,而与南瓜等抗冷性较差的植物不同 ,这些位点的氨基酸残基可能也与GPAT对底物酰基的选择性有关。
Glycerol-3-phosphate acyltransferase (GPAT) is closely related to the plant chill-resistance. Squash ( Cucurbita moschata ) and figleaf gourd ( Cucurbita ficifolia ) are close in taxonomy but very different in chill-resistance. The cloning and sequence of figleaf gourd and squash give us a chance to study the relationship between GPAT and plant chill-resistance in molecular level, from a few different amino-acid residues compared with the two GPAT amino-acid sequences. Among thirteen different amino acid residues between squash and figleaf gourd, three of them are the same as those of chill-resistant plants, Arabidopsis thiliana, redflow ( Carthamus tinctorius ), pea ( Pisum sativum ) and spinach ( Spinacia oleracea ), which may related to the chill-resistant difference of squash and figleaf gourd. Compared with the GPAT amino acid among squash, figleaf gourd, Arabidopsis thaliana, spinach, pea, redflow, etc., some sites of amino acid are the same in chill-resistant plants but different from squash, figleaf gourd and cucumber. These amino acids may also related to the selectivity of GPAT to different fatty acids in different plants.
出处
《云南植物研究》
CAS
CSCD
2000年第2期169-174,共6页
Acta Botanica Yunnanica
基金
国家自然科学基金
云南省自然科学基金
云南省教委基金资助项目
