摘要
以非洲菊叶柄基部的愈伤组织为材料,通过农杆菌介导法,建立高效稳定的遗传转化体系.结果表明,OD600值为0.3的菌液浓度,侵染时间8 min,36 h的共培养,延迟筛选2 d有利于获得较高的转化效率.通过农杆菌介导,持续筛选和PCR检测,美洲商陆蛋白(PAP)基因成功转入非洲菊幼苗.
A highly efficient and stable Agrobacterium-mediated genetic transformation system for Gerbera jamesonii Bolus was established using the calli from the petiole base of the plant as the starting materials for transformation.The highest transformation frequency was obtained in this study with an OD600 of about 0.3 for Agrobacterium,an infection duration of 8 min,co-culture for 36 hours,and detention screening for 2 days.PCR proved that the pokeweed antiviral protein（PAP） gene had been successfully integrated into G.jamesonii Bolus.
出处
《西南师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2010年第3期76-80,共5页
Journal of Southwest China Normal University(Natural Science Edition)
关键词
美洲商陆
根癌农杆菌
遗传转化
非洲菊
pokeweed
Agrobacterium tumefaciens
genetic transformation
Gerbera jamesonii Bolus
