摘要
依据国外报道的南瓜甘油－３－磷酸转酰酶（ＧＰＡＴ）基因的ｃＤＮＡ序列合成相应引物，用ＲＴ－ＰＣＲ技术，成功地分离了黑子南瓜（Ｃｕｃｕｒｂｉｔａｆｉｃｉｆｏｌｉａ）ＧＰＡＴ基因的ｃＤＮＡ片段，并亚克隆到了ｐＧＥＭ－Ｔ载体系统的多克隆位点上，序列分析表明黑子南瓜ＧＰＡＴ基因的ｃＤＮＡ序列及递推的氨基酸序列与南瓜（Ｃｕｃｕｒｂｉｔａｍｏｓｃｈａｔａ）相比分别具有９８％和９６５％的同源性。在１１８８ｂｐ中有２２个核苷酸发生变化。
According to the cDNA sequence of GPAT from squash( Cucurbita moschata )reported in 1988,using RT-PCR technical, we isolated the cDNA encoding a glycero1-3-phosphate acyltransferase(GPAT)from figleaf gourd( Cucurbita ficifolia )cotyledons and subcloned in pGEM-T vector system. The cDNA and the deduced amino acid sequence of figleaf gourd GPAT have 98%and 96 5% homologous with squash respectively. Among the deduced amino acid residues thirteen of them are different from that of squash.
出处
《云南植物研究》
CSCD
1999年第2期139-143,共5页
Acta Botanica Yunnanica
基金
国家自然科学基金
云南省自然科学基金
关键词
黑子南瓜
甘油
磷酸酰基转移酶
CDNA
Cucurbita ficifolia ,Glycerol -3-phosphate acyltransferase, cDNA
