摘要
目的通过观察叶酸和维生素B12对人神经母细胞瘤株SH-SY5Y细胞生长的影响,探讨叶酸和维生素B12对神经细胞的保护作用。方法体外培养SH-SY5Y细胞,分为对照组、叶酸组(1.875 mg/L)、维生素B12组(800μg/L)、叶酸(1.875 mg/L)+维生素B12(800μg/L)组,进行细胞形态观察、细胞计数分析以及测定各组细胞的噻唑蓝(thiazolyl blue,MTT)代谢率、乳酸脱氢酶(lactate dehydrogenase,LDH)漏出率。结果与对照组相比,叶酸组、维生素B12组、叶酸+维生素B12组的细胞计数和MTT代谢率升高(P<0.05),LDH漏出率降低(P<0.05),在细胞形态上,叶酸组、维生素B12组、叶酸+维生素B12组明显好于对照组,表现为细胞胞体饱满、存活细胞数目增多、贴壁良好、突起延长。结论叶酸、维生素B12能促进神经细胞生长,降低神经细胞死亡率,从而达到保护神经元的作用。
Objective To observe the protective effect of folic acid and vitamin B12 on human neuroblastoma cell line SH-SYSY cell growth in vitro. Methods SH-SYSY cells cultured in vitro, divided into control group, the folic acid（ 1. 875 mg/L） group, vitamin B12 （800 μg/L） group, folic acid （1. 875 mg/L） ＋ vitamin B12（800 μg/L） group. Cell morphology, cell count analysis, thiazolyl blue （MTT） metabolic rate, and lactate dehydrogenase（LDH） leakage rate of each group were detected. Results Compared with the control group, cell count and MTT metabolic rate of folic acid group, vitamin B12 group, folic acid＋vitamin B12 group increased（ P〈0.05 ）, and LDH leakage rate decreased（P〈0.05）. Compared with the control group, cell morphology were much better in those groups, cell body more plump, the number of surviving cells increased, adherence was good and neurite extension could be found. Conclusion Folic acid and vitamin B12 can promote nerve cell growth, reduce nerve cell death, in order to achieve the protection of neurons.
出处
《首都医科大学学报》
CAS
2012年第6期776-778,共3页
Journal of Capital Medical University
基金
北京市自然科学基金(7082043)
首都医学发展科研基金(2007-3108)
北京市卫生系统高层次卫生技术人才计划资助项目(2009-3-64)~~
