摘要
目的 对小鼠腹腔巨噬细胞及人精子的甘露糖受体 (MR)进行超微结构定位。 方法 采用甘露糖基化牛血清白蛋白 (DMA)结合 10 nm胶体金制备探针 DMA- G1 0 ,以此探针标记小鼠腹腔巨噬细胞和获能前后的人精子 ,并用透射电子显微镜观察。 结果 所制备的 DMA- G1 0 具有甘露糖基结合特性。小鼠腹腔巨噬细胞可分为明、暗两种类型。“暗细胞”不含 MR;“明细胞”细胞膜表面含 MR,该受体与 DMA- G1 0 结合后内化入细胞内。人精子发生顶体反应后其顶体内膜、顶体小泡或赤道带含 MR,顶体完整的精子其 MR未被标记。 结论 小鼠巨噬细胞MR位于胞膜上 ,人精子 MR可能存在于顶体内。胶体金亲和细胞化学法进行细胞的甘露糖受体定位具有可靠性 ,在一定范围内可用于其他细胞 MR的定位。
Objective\ To locate mannose recepter(MR) of mouse macrophage and human sperm on ultrastructural level.\ Methods\ D\|Mannosylated\|BSA(DMA,A8303) absorbed on colloidal gold particles of size 10 nm as a probe was used to detect MR of macrophage from mouse abdominal cavity and human sperm before or after capacitation, and then visualized by transmission electron microscopy.\ Results\ Complex probe of DMA\|G\-\{10\} can combined mannose residues specifically.\ Macrophages were divided into two subtypes, 'light' and 'dark'.\ There was no receptor observed in 'dark' macrophage.\ MRs distributed on the surface of plasma membrane of 'light' macrophage and some entered the cells by endocytosis.\ In human sperm, MRs were labeled on the surface of inner acrosomal membrane or acrosomal vesicles of acrosome after capacitation in vitro, while in some sperm, the gold granules concentrated in a narrow band on the IAM of equatorial segment after acrosome reaction had completely finished.\ MRs were not detected in acrosome intact spermatozoa.\ Conclusion\ MR of mouse microphage was on plasma membrane,while MR of human sperm might be in acrosome.\ Affinity\|gold cytochemistry for detection of MR reported here was reliable and may be used on other cells study. \;
出处
《福建医科大学学报》
2000年第2期110-114,共5页
Journal of Fujian Medical University
基金
福建省科技三项费资助!项目 ( K940 3 5 )
关键词
甘露糖受体
巨噬细胞
精子
亲和细胞
化学电镜
mannose receptor
colloidal gold
macrophage
spermatozoa
microscopy,electron,scanning
mice
