摘要
目的:研究基质细胞衍生因子-1α(stromal cell-derived fac-tor-1α,SDF-1α)对体外培养的人类视网膜色素上皮(hu-man retinal pigment epithelium,hRPE)细胞增殖的影响。方法:应用MTT法研究SDF-1α对体外培养的人RPE细胞增殖的影响;用细胞化学染色法检测添加SDF-1α前后人视网膜色素上皮细胞内细胞增殖核抗原(proliferatingcell nucler antigen,PCNA)的表达情况。结果:MTT法表明SDF-1α可促进体外培养的hRPE细胞的增殖(P<0.05);细胞化学电镜下观察发现:添加SDF-1α前hRPE细胞未表达或低表达PCNA,而添加SDF-1α后细胞高度表达PCNA。结论:细胞因子SDF-1α可促进体外培养的hRPE细胞增殖。
AIM： To investigate the effect of stromal cell-derived factor-It（（SDF-la） on the proliferation of human retinal pigment epithelium （hRPE） in vitro.
METHODS： MTT was used to investigate the effect of SDF-la on the proliferation of hRPE in vitro. Cytochemical staining method was used to detect the expression of proliferating cell nuclear antigen （PCNA） in hRPE with SDF-1α.
RESULTS. MTT showed that SDF -1α could stimulate the proliferation of hRPE in vitro（ P〈 0. 05）, Cytochemistry results indicated that there were no or low expression of PCNA before adding SDF-1α, but the expression became higher after adding SDF-1α observed under electric mirror.
CONCLUSION. Cytokine SDF-1α can stimulate the proliferation of hRPE cultured in vitro.
出处
《国际眼科杂志》
CAS
2008年第10期2001-2004,共4页
International Eye Science
基金
中国卫生部科研基金资助项目(No.WKJ2005-2-013)
福建省重大科研项目(No.002Y002)
福建医科大学教授发展基金课题(No.2006-js6033)~~
