摘要
目的 :探讨喉鳞状细胞癌 Hep- 2细胞系染色体畸变及其核型特征 ,认识喉癌的细胞遗传学改变与其发病机制的相关性。方法 :应用常规和高分辨 G显带方法进行核型分析。结果 :Hep- 2细胞系的染色体数目变化于 5 4～ 84条之间 ,众数为 6 9～ 74条。可识别其结构的稳定的标记染色体为 13条 ,部分核型中存在双微体 ,是基因扩增的细胞遗传学标志。结论 :喉癌 Hep- 2细胞系属超三倍体细胞 ,存在染色体数目和结构畸变并具有稳定的标记染色体及基因扩增。结构畸变涉及易位、缺失和等臂染色体。染色体断裂重接导致染色体重排 ,3、5、6和
Objective:Our purpose was to study the chromosomal aberration and characteristics of the karyotype of cell line of laryngeal carcinoma(Hep 2),and to explore the relationship between cytogenetic changes and pathogenesis in laryngeal carcinoma. Methods:An karyotype analysis of Hep 2 was performed with routine and high resolution G banding technique. Results:The chromosomal number of the Hep 2 ranged from 54 to 84 ,and the chromosomal model ranged from 69 to 74. Among the abnormal chromosomes,13 were marker chromosomes with distinguishable and stabile structure. DM existed in some of the karyotypes,and it was the cytogenetic marker of the gene amplification. Conclusion:There were aberration of number and structure of the chromosomes as well as marker chromosomes and gene amplification in Hep 2 in which belonged to supertrisomy. The structure aberration of chromosomes included the translocation,deletion,and isochromosome. The break and rejoint of the chromosome resulted in its rearrangement. The deletion and rearrangement of the 3,5,6 and 8 chromosomes were the characteristic changes of the Hep 2.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2000年第3期167-169,共3页
Journal of China Medical University
基金
辽宁省自然科学基金!972 2 2 5
国家自然科学基金!3 9770 794
关键词
喉癌
鳞状细胞癌
HEP-2细胞系
染色体畸变
laryngeal carcinoma cell line
high resolution G banding technique
chromosomal aberration
translocation
deletion
isochromosome
