摘要
目的 检测肺癌组织中 Ｆ Ｈ Ｉ Ｔ 基因的异常表达。方法 收集手术切除２１ 例肺鳞癌、１０例肺腺癌及对应的正常肺组织标本， 应用逆转录巢式聚合酶链反应（ Ｒ Ｔ Ｐ Ｃ Ｒ） 及 Ｄ Ｎ Ａ 测序技术检测 Ｆ Ｈ Ｉ Ｔ基因的表达情况。结果 ３１ 例正常肺组织中均有 Ｆ Ｈ Ｉ Ｔ 基因的完整表达，１５ 例（４８ ％ ） 肺癌标本中存在 Ｆ Ｈ Ｉ Ｔ基因缺失；肺鳞癌中缺失率为５７ ％ （１２／２１） ，肺腺癌中缺失率为３３ ％ （３／１０） 。经测序证实， Ｆ Ｈ Ｉ Ｔ基因ｍ Ｒ Ｎ Ａ 异常转录本为其多个外显子缺失所致，并全部缺失 Ｅ５ ，在１ 例肺鳞癌中密码子９８存在同义点突变。结论 位于３ｐ１４２ 的 Ｆ Ｈ Ｉ Ｔ 基因在国人肺癌中存在较高的缺失率， 提示 Ｆ Ｈ Ｉ Ｔ 基因为一侯选抑癌基因， 在肺癌的发生发展中起重要作用。
Objective To determine whether the FHIT gene at 3p14 2 is abnormal in Chinese lung cancers Methods Matched normal and cancerous tissues from 21 cases of primary pulmonary squamous cell carcinoma (SQC) and 10 cases of adenocarcinoma (ADC) were obtained immediately after surgery Total RNA was extracted and the FHIT gene was detected by RT PCR and DNA sequencing technology Results Normal sized FHIT transcript was detected in all 31 cases of normal matched tissues Aberrant transcripts were observed in 15(48%) of 31 cases of cancerous tissues, 57%(12/21) in SQC and 33%(3/10) in ADC, respectively All the abnormal transcripts observed lacked exon 5,the first coding exon The sequence analyses of the aberrant cDNAs revealed deletions of various regions between exon 4 and 10 A synonymous mutation in E8,codon98CAT(H)→CAC(H), was found in a SQC Conclusion The high deletion rate of the FHIT gene in Chinese lung cancer supports the hypothesis that the FHIT gene alteration is involved in tumorigenic development of human neoplasm
出处
《中华医学杂志》
CAS
CSCD
北大核心
1999年第8期592-595,共4页
National Medical Journal of China
