摘要
通过对目前常用的黄曲霉毒素B1(AFT B1)检测方法的研究,开发出一种高效、低耗的检测方法。该方法采用甲醇-水(7∶3)溶液提取食用油中的黄曲霉毒素B1,提取液经冷冻过滤后,通过配有柱后衍生系统和荧光检测器(FLD)的高效液相色谱(HPLC)分析。该方法最低检出限为0.4μg/kg,相对标准偏差为1.25%～2.42%,加标回收率为93.47%～96.78%。该方法与其他仪器检测方法相比不用任何净化小柱,缩短了检测时间,降低了检测成本。
A high efficient and low -cost detection method was developed by the research on the currently used detection methods of aflatoxin B1 （ AFT B1 ）. AFT B1 was extracted from edible oil with methanol - water （7：3 ）, and the extract was analyzed by high performance liquid chromatography （HPLC） coupled with post column derigatization system and fluorescence detection （FLD） after freeze -filtering. The de- tection limit was 0.4 μg/kg, relative standard deviation was 1.25% -2.42% and standard recovery was 93.47% -96.78%. Compared with other instrument detection methods, this method had no clean -up column, and made the detection time shorter and the cost lower.
出处
《中国油脂》
CAS
CSCD
北大核心
2012年第12期43-46,共4页
China Oils and Fats
