摘要
目的：建立一种快速、灵敏、简便的高效液相色谱（HPLC）同时测定食品中黄曲霉毒素（AFT）B1、B2、G1和G2的方法。方法：样品经振荡提取，多功能净化柱净化，三氟乙酸衍生后，选用ZORBAX XDB-C18分离柱，用乙腈-水（23＋77）作流动相，于20℃以下等度洗脱，经荧光检测器测定后，以保留时间定性，用峰面积或峰高定量。结果：20分钟内可完成AFr4种组分的分离。检出限分别是，B1为0．042ng，B2、G1和G2为0．08ng。回收率为83．4％～103．1％，r〉0．9995。结论：经振荡提取、多功能净化柱净化后，用荧光检测器同时测定食品中黄曲霉毒素（AFT）B1、B2、G1和G2的高效液相色谱方法快速、灵敏、简便。
Objective： To establish a rapid, sensitive and simple mettwcl for the simultaneous determination of aflatoxin B1, B2, G1, G2 in foods by high performance liquid chromatography with multifunctional cleaning column.Method： Sample was extracted and cleaned - up with the multifunctional cleaning column. The purified solution was derivatized with TFA and isolated by ZORBAX XDB-C18 separating column, then eluted with 23% acetonitrile at 20℃. Aflatoxins were detected and quantitated by HPLC with fluorescence detection. Results： The four toxins could be separated in 20 minutes and the detection limit was 0.042ng for aflatoxin B1, and 0.08ng for aflatoxin B2, G1, G2.Conclusion： The mettwcl has been successfully applied to determination of aflatoxin B1, B2, G1, G2 in foods.The recoveries of aflatoxins was in the range of 83.4% -103.1%, the correlation coefficient〉0.9995.
出处
《现代预防医学》
CAS
北大核心
2006年第5期790-791,共2页
Modern Preventive Medicine
