摘要
采用自行设计搭建的激光诱导荧光(LIF)-高效毛细管电泳(HPCE)检测平台,建立LIF-HPCE法对食品中的黄曲霉毒素B1(AFB1)进行高灵敏度检测。AFB1在胶束电动毛细管电泳(MECC)模式下分离,LIF检测,375nm激光进行激发,检测波长440nm,操作电压15kV,电流104μA。AFB1在0.5～50μg/kg浓度范围内线性关系良好,r=0.9994;最低检出质量1.7×10-13g(S/N=3),最低定量限5.6×10-13g(S/N=10),方法精密度和重复性的相对标准偏差为5%左右。测定食品中粮油等食品样品,加标回收率为84.1%～96.1%。所建立的方法无需进行衍生反应和荧光标记,快速灵敏,绿色环保,10min左右完成分析,适用于食品中黄曲霉毒素的高灵敏度检测。
With self-made detection platform, a laser induced fluorescence-high performance capillary electrophoresis （LIF- HPCE） method was developed for detecting aflatoxin B1 （AFB1） in food. AFB1 was separated under micellar electrokinetic capillary chromatography （MECC） mode （voltage 15 kV, and current 104 μA）, excited by laser at the wavelength of 375 nm, and detected by LIF at the wavelength of 440 nm. The data showed that the linear relationship of AFB1 was nice （r = 0.9994） in 0.5 to 50 μg/kg concentlation range. The lowest detection limit was 1.7 × 10^-13g （S/N = 3） and the lowest quantitative limit of AFB1 was 5.6 × 10^-13g （S/N=10）. The repeatability and the precision expressed as RSD values are about 5%. The spike recovery was in the range of 84.1% to 96.1% for AFB1 in food. This LIF-HPCE method can complete analysis within 10 min without derivative reaction or fluorescence labeling, and is suitable for high sensitivity determination of aflatoxins in food.
出处
《食品科学》
CAS
CSCD
北大核心
2009年第10期135-139,共5页
Food Science
基金
科技部重点项目(2006JG003700)
国家“863”计划项目(2007AA10Z427)
国家“973”计划项目(2009CB118803)
重庆市科委2009年攻关项目
西南大学博士基金项目(SWUB2007044)
关键词
黄曲霉毒素B1
激光诱导荧光
高效毛细管电泳
胶束电动毛细管电泳
aflatoxin B1
laser induced fluorescence （LIF）
high performance capillary electrophoresis （HPCE）
micellar electrokinetic capillary chromatography （MECC）
