摘要
目的 探讨转化生长因子 β(TGF β)对体外培养的牛眼晶状体上皮细胞凋亡的作用。 方法 MTT法测定细胞经不同浓度TGF β作用后的增殖情况 ;以TUNEL技术研究TGF β作用不同时间后细胞凋亡的变化。 结果 TGF β可显著抑制晶状体上皮细胞的增殖 ,抑制率高达 35 %。原位凋亡测定显示TGF β作用 30min至 8h可明显诱导晶状体上皮细胞凋亡 ,阳性细胞核由边缘着色逐渐变为整个核均匀着色。结论 转化生长因子 β抑制晶状体上皮细胞增殖的一个重要途径是诱导细胞凋亡。
ObjectiveTo study the effects of transforming growth factor β (TGF β) on inducing apoptosis in bovine lens epithelial cells (LECs) in vitro.MethodsThe third passage LECs were seeded in the presence of different concentrations of TGF β 2.MTT assay was used to assess the cell proliferation.Apoptosis induced by TGF β 2 was studied by TDT mediated digoxigenin dUTP nick end labeling (TUNEL) technique.ResultsTGF β significantly inhibited the proliferation of LECs,and the maximal inhibitory rate was 35%.In situ apoptosis detection showed that both 0 01?ng/ml and 0.1 ng/ml TGF β could induce apoptosis apparently in LECs.By light microscopy,the nucleus of normal cells were stained green,while that of apoptotic cells were stained yellow or brown.The percentage of apoptotic cells increased more than two times when LECs co cultured with TGF β.ConclusionIt is suggested that inducing apoptosis may be one of the important pathways of transforming growth factor β in inhibiting the proliferation of lens epithelial cells.
出处
《眼科研究》
CSCD
2000年第2期114-116,共3页
Chinese Ophthalmic Research
