摘要
以百脉根品种—里奥为材料,通过不同组培配方培养基培养后,观察萌芽、生根和生长情况,确定用于百脉根一次性生根成苗的组织培养快繁方法和培养程序,其最佳培养基配方为:MS+IBA 0.1 mg/L+NAA 0.05 mg/L+蔗糖30 g+琼脂8 g,pH 5.5～5.8。该配方和程序具有培养周期短、操作简便、成本较低、易于操作等特点。
After years of research, we have found out a rapid reproduction method which was suitable for Lotus corniculatus L. Used of one-time root into the tissue culture seedling, it abandon traditional three-stage plant tissue culture program, screened the best medium formula （MS ＋ IBA 0.1 mg/L ＋ NAA 0.05 mg/L ＋ 30 g sucrose ＋ 8 g agar, pH 5.5 - 5.8） and nurturing program, this formulation and process has the short-cycle, simple-operation, low-cost and easy-operation etc., in the rapid reproduction of tissue culture technology and program for Lotus corniculatus L. realize factory production to create the possibility.
出处
《热带农业科学》
2012年第12期5-8,共4页
Chinese Journal of Tropical Agriculture
关键词
百脉根
组织培养
快繁技术
Lotus Corniculatus L.
tissue culture
rapid propagation technique
