摘要
【目的】将H5N1亚型禽流感病毒血凝素(AIVHA)基因转入牧草植物中,利用豆科牧草作为植物生物反应器来生产禽流感抗原蛋白,探索研制禽流感转基因植物可饲用疫苗的可行性。【方法】以百脉根子叶柄外植体作为转化受体,通过农杆菌介导法将AIVHA基因导入百脉根,子叶柄外植体经过共培养、筛选分化、再生,得到抗性植株。对抗性植株进行了PCR、RT-PCR检测和Western blot分析。【结果】证明AIVHA基因已经导入到百脉根基因组中,在核酸水平和蛋白水平都得到了表达。【结论】利用百脉根表达禽流感抗原蛋白是可行的。
[ Objective ] Avian influenza virus （AIV） hemagglutinin （H5N1） gene was transformed into the Lotus corniculatus. Legume forage was used as a plant bioreactor to produce the H5N1 antigen protein in order to investigate the feasibility of developing edible transgenic plant vaccine of AIV. [Method] Taking the cotyledon petiole explant as the transformation receptors, hemagglutinin gene of avian influenza was transformed into Lotus corniculatus by Agrobacterium-mediated transformation. After co-culture, selective differentiation and regeneration, resistant plants were obtained. Resistant plants were detected by PCR, RT-PCR and western blotting. [Result] The results have proved that hemagglutinin gene has been transformed into the genome of Lotus corniculatus. The hemagglutinin gene has been expressed on protein level and nucleic acid level in the transgenic plants and the result is better than the control. [Conclusion] It is feasible to use plant bioreactor to produce AIV H5N1 antigen protein in the legume forage.
出处
《中国农业科学》
CAS
CSCD
北大核心
2008年第1期303-307,共5页
Scientia Agricultura Sinica
基金
国家重点基础研究发展计划(“973”计划,2007CB108902)
关键词
禽流感
血凝素
转化
百脉根
Avian influenza vires
Hemagglutinin
Transformation
Lotus corniculatus L.
