摘要
目的:研究蒙药光明盐四味汤散对大鼠动脉粥样硬化氧化应激的影响,并探讨其对HO-1表达影响的作用机制,为临床用药提供实验依据。方法:Wistar大鼠40只,随机分为5组,造模的同时预防性给药。采用全自动生化仪检测血清中总胆固醇(TG)、甘油三酯(TC)、低密度脂蛋白(LDL)和高密度脂蛋白(HDL),TAB法测定MDA(丙二醛)含量,黄嘌呤氧化法测定SOD(超氧化物歧化酶)活性,ELISA方法测定(血红素加氧酶-1)HO-1活性,光镜下观察主动脉的病理学形态。结果:(1)蒙药高、低剂量组能升高SOD活性、降低MDA含量,差异有统计学意义(P≤0.05)。(2)蒙药高、低剂量组能降低血清HO-1含量,差异有统计学意义(P≤0.05)。结论::高脂饲料加一次性大剂量维生素D3负荷共同作用可成功建立大鼠早期AS模型。蒙药光明盐四味汤散具有降血脂、抗脂质过氧化等作用,对AS早期氧化损伤有一定的预防作用,为进一步探索早期预防动脉粥样硬化发病提供了理论依据。
Objective：To study the effect of rats＇ atherosclerosis oxidative stress with Mongolian Medicine Bright Sah-4 Decoction,and investigate the influence the expression of mechanism of action on the expression of Ho-1, which is in order to provide the evidence for clinical medication. Method： Forty Wistar rats were divided into 5 group randomly. Giving the medical when copy animal model. Test the contents of total cholesterol, triglyceride, low density lipoprotein, high density lipoprotein by automatic biochemistry analyzer,test MDA in serum by TAB method, tests the activity of SOD by xan thinoxidase method and test the activity of HO- 1 by ELISA method, and observe the pathological shape of aorta hy optical microscope. Results： 1. The high and low dosage of Mongolian Medicine group can increase the SOD activity, and reduce the MDA content. The difference has the statistical significance （P≤0.05） ;2. The high and low dosage of Mongolian Medicine group can decrease the HO-1 content. The difference has the statistical significance （ P ≤ 0.05 ）. Conclusion： The early AS rat model can besuccessfully established with the coaction of high-fat feed stuff once add the megadose of vitamin D3 load. Mongolian Medicine Bright Salt-4 Decoction has the function of hypolipidemic and anti-lipid peroxidation. It has the function of preventative effect for the AS early oxidative damage, and it provides the theoretical foundation of further quest for early prevention of atherosclerosis onset.
出处
《内蒙古医学院学报》
2012年第5期356-360,共5页
Acta Academiae Medicinae Neimongol
