摘要
本文应用双PAP法对二甲基亚硝胺(N-nitrosodjmethylamine,NDMA)诱导大鼠DNAO^6—甲基鸟嘌呤(O^5—methylguanine,O^6—mG)进行了研究。分别给大鼠一次腹腔注射NDMA 1,2,3,5,10,30,50mg/kg,5小时后大鼠产生O^6—mG具有明显的剂量反应关系以及器官和细胞特异性。随着NDMA剂量的增加,产生O^6—mG的器官种类和细胞类型增加,阳性细胞形成O^6—mG的量也增加。小剂量(1—5mg/kg)NDMA仅见肝组织的细胞形成O^6—mG,NDMA剂量达50mg/kg时,肝、肾、肺、气管,食管和鼻咽组织的细胞均检出O^6—mG。
With double PAP technique, N-nitrosodimethylamine (NDMA)- induced O6 - methylguanine(O 6-mG) in the DNA of rat tissues was localized. Fivg hours after a single i. p. injection of NDMA at doses of 1,2, 3,5,10, 30, 50 mg/kg respectively, the production of O 6- mG in the DNA of rats displayed obvious dose-response relationship as well as cell and organ specificity. The number of O 6- mG positive organs, the types of O 6-mG cells and the production of O 6-mG in positive cells increased with the increase of NDMA dose. Among all the organs the liver was the most sensitive one. O 6- mG positive cells were only, found within the hepatic lobules of rats treated with l-5mg/kg NDMA. O6- mG positive cells were found in the liver, kidney, and lung tissues of rats after injection of 10 mg/kg NDMA. when the dose of NDMA reached 50mg/kg, O 6-mG positive cells were observed in the liver, Kidney, lung, traochea, esophagus, and the nasopharynx.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
1991年第4期308-311,共4页
Chinese Journal of Cancer
关键词
二甲基亚硝胺
甲基鸟嘌呤
免疫组化
N-nitrosodimethykmine
O 8-methylguanine
Immunohistoche m istry
