摘要
目的探讨子宫颈病变中TGF/Smads信号通路相关蛋白表达与HPV16感染的关系。方法采用原位杂交法检测维吾尔族妇女慢性子宫颈炎、CIN1～3和子宫颈鳞状细胞癌中HPV16的感染情况,采用免疫组化SP法检测子宫颈病变组织中TGF-β1、Smad4、Runx3蛋白表达。结果 HPV16在慢性子宫颈炎、CIN和鳞状细胞癌组织中的感染率分别为8%、47.62%、62.22%;TGF-β1蛋白在3组子宫颈病变上皮及间质中阳性率分别为0、41.26%、71.11%和12%、61.9%、53.33%,随着子宫颈上皮病变加重表达增强(P<0.01)。Smad4和Runx3在慢性子宫颈炎、CIN和鳞状细胞癌组织的表达呈递减趋势,阳性率分别为80%、69.84%、40%和84%、69.84%、44.44%(P<0.01);在鳞状细胞癌组TGF-β1与HPV16型感染呈正相关(r=0.515,P<0.001),与Runx3呈负相关(r=-0.318,P<0.033),Smad4和Runx3的表达呈正相关(r=0.329,P<0.027)。结论TGF-β1、Smad4、Runx3信号通路相关蛋白表达的改变可能与子宫颈癌的发生、发展有关,而HPV16的感染可能是影响通路分子表达改变的前提条件。
Purpose To explore the relationship between human papillomavirus （HPV16） infection and expression of TGF/Smads sig- nal pathway protein TGF-[31, Smad4, Runx3 in cervical cancer of Uighur women, and to investigate their effect on cervical cancer pro- gression. Methods In situ hybridization and immunohistochemistt7 were performed to detect the expression of HPV16 and TGF-I31, Smad4, Runx3 protein in formalin-fixed and paraffin-embeded cervical tissue specimens from Uighur women with cervicitis, cervical in- traepithelial neoplasia （C1N） and cervical squamous cell carcinoma. Results The positive expression rates of HPVI6, TGF-β1 in chronic cervicitis, CIN and cervical cancer were 8% , 47.62% , 62.22% and 0, 41.26% , 71.11% , respectively. The expression of HPVI6 and TGF-[31 was higher in CIN and cervical cancer than in chronical cervicitis （P 〈 O. 05 ）. The expression of HPVI6 and TGF-β1 was related to the FIC, O stage. TGF-~I expression was found in both cytoplasm and extraeellular stroma, increased when the pathologic grade was getting worse and when the lymphnode had metastasis. The positive expression rates of Smad4 and Runx3 in chro- nic eervieitis, CIN and cervical cancer were 80% , 69.84% , 40% and 84% , 69.84% , 44. 44% , respectively（ P 〈 0. 05 ）. Runx3 was related to lymph node metastasis. In cervical cancer there was positive correlation between HPV16 and TGF-β1 （r =0. 515, P 〈 0. 001 ） , Smad4 and Runx3 also had positive correlation in cervical cancer（ r = 0. 329, P 〈 0. 027 ） , but a negative correlation was found between TGF-β1 with Runx3 （r = -0. 318, P 〈0. 033）. Conclusions The changes in the expression of TGF-I31 , Smad4 and Runx3 in cervical lesions might be related to the pathogenesis and progression of CIN and cervical cancer in Uighur women, and HPV16 infection may be a tri^er factor affectin~ the molecular signal pathway.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2012年第12期1356-1360,共5页
Chinese Journal of Clinical and Experimental Pathology
基金
新疆自然科学青年基金(2012211B10)
