摘要
为更好地利用大量的废啤酒酵母资源,研究了废酵母在H2O2氧化胁迫下形成的活酵母细胞衍生物(LYCD)的活性,优化了胁迫条件.研究结果表明:废酵母细胞经终浓度0.2mmol/LH2O2胁迫15min形成的促细胞生长活性最高,超氧化物歧化酶(SOD)活力是对照的18倍,丙二醛(MDA)含量约为对照的40%;SOD的活力与MDA含量呈现大致的反比关系;胁迫30min,LYCD中还原型谷胱甘肽(GSH)是对照的2.7倍;活性物质的最高值并未在相同的胁迫条件下出现.
For the purpose of utilizing abundant waste yeast resource, the activity of living yeast cell derivative （LYCD） from yeast cell under H2O2 oxidative stress was studied. Results showed that： the activity of LYCD reached peak level after 15-minute stress with final concentration 0.2 mmol/L H2O2; Superoxide dismutase （SOD） activity was 18 times as high as that of the control, whereas malonaldehyde （MDA） concentration was about 40% of that in control; SOD activity and MDA concentration presented inverse ratio relationship; Reduced glutathione concentration was 2.7 times as high as that of control after 30-minute stress with final concentration 0.2mmol/L H2O2; The active substances in LYCD did not simultaneously reach the highest level.
出处
《常熟理工学院学报》
2012年第10期65-69,81,共6页
Journal of Changshu Institute of Technology
基金
江苏省高校自然科学研究计划资助项目"新型非降解技术提取啤酒酵母β-D葡聚糖的工艺研究"(08KJD180006)
