摘要
目的研究啤酒废酵母多糖对小鼠免疫功能的影响。方法以啤酒废酵母为材料,用柠檬酸法提取酵母多糖,苯酚-硫酸法测定多糖含量,纸层析鉴定多糖组分。随机将18月龄的雄性小鼠分为2组,一组每天定时灌胃1%多糖1.0 mL,一组灌胃等量生理盐水,连续30 d。结果2组小鼠的碳颗粒校正廓清指数、肝脏和脾脏指数以及组织匀浆中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性和丙二醛(MDA)含量有显著差异。结论酵母多糖可促进小鼠免疫器官生长,增强小鼠巨噬细胞的吞噬能力;可显著提高SOD、CAT活性,减少肝脏、脾脏中MDA的含量。
Objective To study the effect of waste beer yeast polysaccharide on immunologic function in mice. Methods With waste beer yeast as the material, the yeast polysaccharide was extracted with citric acid. The polysaccharide content was determined with phenol-sulfuric acid method and component analysis was performed with paper chromatography. The male mice at the age of 18 months were randomly divided into two groups. One group was lavaged with 1.0 mL of 1% polysaecharide for 30 days, the other group was treated with equivalent normal saline for 30 days. Results The corrected carbon clearance value, liver and spleen index and the activities of superoxide dismutase（SOD） and catalase（CAT） as well as the content of malondialdehyde（MDA） in tissue homogenate were significantly different between the two groups. Conclusion Yeast polysaccharide can promote the growth of immune organs, enhance macrophage phagocytosis, significantly increase the activities of SOD and CAT, and meanwhile reduce the MDA content in liver and spleen in mice.
出处
《食品与药品》
CAS
2009年第4期26-28,共3页
Food and Drug
关键词
酵母
多糖
小鼠
免疫功能
yeast
polysaccharide
mouse
immunologic function
