摘要
目的:研究中药山豆根(SSCC)提取物对体外培养的人肝癌细胞的抑癌活性。方法:SSCC经粉碎、浸提、超速离心、减压浓缩、冷冻干燥得SSCC提取物。人肝癌SMMC-7721细胞经SSCC提取物处理不同时间后,以MTT法测SSCC提取物对人肝癌细胞线粒体代谢活性的影响,以细胞记数法观察了其对细胞增殖的影响。结果:1经SSCC提取物处理48h,其A570光吸收降为对照组的62.1%,72h为9.64%;2SSCC提取物处理7d后,人肝癌细胞数明显低于对照组;3经SSCC提取物处理24h后,在更换为正常培养基恢复培养过程中,细胞数呈现缓慢增长;4经SSCC提取物处理24h后其有丝分裂指数为对照组的50.6%。结论:中药SSCC提取物能抑制体外培养的人肝癌细胞的增殖,降低线粒体代谢活性。
Objective:To dimonstrate the effects of the extracts from Sophora Subprostrata chun et T chan(SSCC) on the proliferation and mitochondria metabolism of human hepatoma SMMC 7721 cells.Methods:After the test of dose dependent experiment,human hepatoma cells were treated with SSCC extracts at dose of 250 μg/ml for 48 h and mitochondria metabolism activity was detected by MTT method.The experiments of effects of SSCC extracts on the proliferation were performed with the treatments of SSCC extracts at dose of 500 μg/ml for 7 d and the proliferation of human hepatoma cells were also examined after treated with SSCC extracts for 24 h and then replaced by cultured in normal media(containing 10% FCS) for 72 h.Result:(1)SSCC extracts could significantly decrease the activity of mitochondria metabolism,and the A570 absorption in the treatment group was only 62.1% of that in control group after 48 h treatment and 9.64% after 72 h treatment.(2)The human hepatoma cell number in the treatment group was much lower than that in control groups during the two groups were treated for seven days.(3)Had been treated for 24 h then cultured in normal media for 72 h,the human hepatoma cells slowly increased in number;(4)After treated for 24 h,the mitosis index in the treatment group was only 50.6% of that in the control group.Conclusion:SSCC extracts could effectively inhibit the proliferation and mitochondria metabolism activty of human hepatoma cells. [
出处
《山东中医药大学学报》
2000年第1期62-64,共3页
Journal of Shandong University of Traditional Chinese Medicine
基金
山东省教委资助! ( J97K0 9)
