摘要
目的探讨刺梨汁对环磷酰胺合并60Co致骨髓抑制小鼠造血功能的影响,研究其促进血细胞上调的作用机制。方法双抗体夹心法检测外周血常规、骨髓基质中红细胞生成素(EPO)、血小板生成素(TPO)、粒细胞生长因子(GCSF)的量。结果刺梨汁能明显改善骨髓抑制小鼠外周血常规,能有效平衡微环境中TPO的量,改善EPO的表达,对骨髓抑制小鼠骨髓细胞上清中GCSF有明显的正调控作用。结论刺梨汁能提高骨髓抑制小鼠外周血血细胞和骨髓有核细胞计数,可促进骨髓抑制小鼠骨髓细胞从G0/G1期进入增殖周期,并能有效调节骨髓微环境中TPO、EPO、GCSF的表达,从而促进骨髓抑制小鼠的造血功能,可作为一种治疗贫血的辅助保健药物。
Objective To investigate the effect of Rosa roxburghii juice（RRJ） on the hematopoiesis in myelosuppressive mice induced by cyclophosphamide merged 60Co and to study the mechanism for making blood cells upregulating.Methods The peripheral blood,bone marrow stromal erythropoietin（EPO）,thrombopoietin（TPO）,and granulocyte growth factor（GCSF） contents were detected by double antibody sandwich method.Results RRJ could significantly improve the peripheral blood of myelosuppressive mice,effectively balance the amount of TPO in the microenvironment,improve the expression of EPO,and significantly up-regulate GCSF in the supernatant of mouse bone marrow cells.Conclusion RRJ could increase the peripheral blood cells and bone marrow nucleated cells in myelosuppressive mice,promote the bone marrow cells in myelosuppressive mice from the G0/G1 into the proliferation cycle,effectively regulate the expressions of TPO,EPO,and GCSF in the bone marrow microenvironment,thereby promote the hematopoietic function of myelosuppressive mice,as a health drug for anemia.
出处
《药物评价研究》
CAS
2012年第6期435-438,共4页
Drug Evaluation Research
关键词
刺梨汁
骨髓抑制
造血功能
细胞因子
Rosa roxburghii juice（RRJ）
bone marrow suppression
hematopoietic function
cytokines
