摘要
目的:考察四物汤对放射线致血虚证小鼠的生血作用及对骨髓Epo,G-CSF基因表达的影响,为阐明四物汤补血作用的分子机制提供依据。方法:采用3.5Gy60Coγ射线全身1次性照射,制备小鼠血虚证模型;检测外周血象;骨髓集落培养观察并计数CFU-GM,BFU-E,CFU-E,CFUmix;RTPCR检测骨髓Epo和G-CSF基因表达。结果:四物汤显著升高放射线致血虚证小鼠外周血白细胞数量和骨髓CFUGM,BFUE,CFUE,CFUmix数量,上调骨髓Epo和GCSF基因表达。结论:四物汤促进骨髓细胞Epo和G-CSF基因的表达可能是其生血作用的机制之一。
Objective： To observe the blood enriching function of Siwu Tang and its effect on Epo and G-CSF gene expressions in bone marrow of blood deficiency mice, and thus provide the basis for understanding the molecular mechanism of blood enriching function of Siwu Tang. Method： The animal model of blood deficiency were established in the mice by using 3.5 Gy^60Co γ-ray. Peripheral blood cells were analyzed and CFU-GM, BFU-E, CFU-E and CFU-mix were counted in bone marrow colony cultured. Both Epo and G-CSF gene expressions inbone marrow were measured with RT-PCR. Result： Siwu Tang significantly increased the number of peripheral blood cells and the amount of CFU-GM, BFU-E, CFU-E and CFU-mix in bone marrow and enhanced Epo and G-CSF gene expression in bone marrow in the mice with blood deficiency. Conclusion： The promotion of Epo and G-CSF gene expressions in bone marrow may be one of the mechanisms underlying the blood enriching function of Siwu Tang decoction.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2005年第15期1173-1176,共4页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(30271617)
军队"十五"重点项目(01Z019)
北京市"二四八"重大创新工程项目(H010210220113)
关键词
四物汤
辐射
血虚证
造血祖细胞
造血生长因子
骨髓
逆转录-聚合酶链反应
Siwu Tang
irradiation
blood deficiency
hematopoietic progenitor cell
hematopoietic growth factors
bone marrow
RT-PCR
