摘要
以初始诱导得到的山苍子[Litsea cubeba(Lour)Pers]幼苗进行继代培养试验。试验共设计3种培养基:改良MS+6-BA 0.5mg/L+NAA 0.2mg/L、改良MS+6-BA 0.8mg/L+NAA 0.2mg/L、改良MS+6-BA 1.0mg/L+NAA 0.2mg/L,2种切割方式,进行4代培养,研究生长调节剂、继代次数和切割方式对山苍子继代增殖的影响。结果以改良MS+6-BA 1.0mg/L+NAA 0.2mg/L培养基比较适宜山苍子的继代培养,当NAA浓度为0.2mg/L时,增殖率随6-BA浓度的增加而增大,愈伤组织逐渐减少,6-BA浓度为1.0mg/L时增殖率最高,苗木生长效果最好;继代到第4代时,山苍子增殖率明显高于前3代,增殖苗长势良好有活力;以2～3个丛芽块的接种方式接种效果优于单芽接种,增殖率可达到3.57,芽苗叶色浓绿效果最好。
The materials of Litsea cubeba from initial culture were used to successive transfer culture. The test was designed using 3 kinds of medium： modified MS＋0.5 mg/L of 6-BA＋0. 2 mg/L of NAA,modified MS＋0.8 mg/L of 6-BA ＋0.2 mg/L of NAA,and modified MS＋1.0 mg/L of 6-BA＋0.2 mg/L of NAA,2 kinds of cutting way,and for 4 generations to culti- vate,in order to analyze the proliferation influence of Litsea cubeba successive transfer culture in different growth regulator, successive transfer times and cutting way. The results showed that modified MS＋1. 0 mg/L of 6-BA＋0.2 mg/L of NAA was optimal medium for successive transfer culture. When the concentration of NAA was 0.2 rag/L, the rare of proliferation in- creased with the increasing of 6-BA concentration,while the callus gradually reduced; when 6- BA concentration was 1.0 mg/L, the proliferation rate reached the highest, and seedling growth effect was the best. Successive transfer to the 4th generation, Litsea cubeba proliferation rate revealed significantly higher than the previous 3 generations,and breeding seedlings grew well dynamic. The effect of inoculation with 2~3 cluster buds was better than that of single graft- ing species,its breeding rate can reach 3.57 and the seedling was well-grown.
出处
《广西科学》
CAS
2012年第4期374-376,共3页
Guangxi Sciences
基金
高峰林业科技园珍贵及乡土树种试验研究项目资助
关键词
山苍子
继代培养
增殖效果
Litsea cubeba, successive transferculture, multiplication
