摘要
为进一步了解猪LPL基因的结构和功能,本研究以苏钟猪为试验对象,利用实时荧光定量PCR方法,分析该基因在苏钟猪5种不同组织(心脏、肝脏、肾脏、皮下脂肪和背最长肌)中RNA水平的表达谱信息;对苏钟猪LPL基因的CDS区域进行了克隆测序,并用生物信息学方法分析了苏钟猪LPL蛋白水平的结构和功能。结果表明:LPL基因在各组织中的mRNA表达丰度为:皮下脂肪>心脏>肾脏>背最长肌>肝脏,其中皮下脂肪的表达量显著高于其他组织(P<0.01),且公猪背最长肌中的表达量显著高于母猪(P<0.01),公猪与母猪间的眼肌面积和瘦肉率性状均差异显著(P<0.05)。猪LPL基因编码含479个氨基酸的蛋白,理论等电点为4.99,具有疏水性,存在信号肽,是分泌型蛋白,与牛、绵羊、家猫等的亲缘关系较近。LPL对苏钟猪的皮下脂肪、心脏、肾脏、肌肉等组织的脂肪沉积有重要影响,可成为苏钟猪肉质改良育种中的重要候选基因。
In order to understand the structure and function of porcine LPL gene, the mRNA expression profile of porcine LPL in five tissues （heart, liver, kidney, subcutaneous fat and lon- gissimus muscle） were examined in Suzhong pigs by Real-time PCR. Besides, the coding se- quences （CDS） of LPL were cloned and sequenced in Suzhong pigs, and molecular structure and function of LPL were analyzed by bioinformatics methods. The results showed that mRNA ex- pression abundance of LPL in different tissues tended to be subcutaneous fat 〉 heart〉kidney〉 longissimus muscle, liver. Among these tissues, LPL expression in subcutaneous fat was signif- icantly higher than that in any other tissues （P〈0.01）, and expression in longissimus muscle of boars was significantly higher than that of sows （P〈0.01）. There was significant difference of eye-muscle area and lean-meat percentage traits between boars and sows （P〈 0. 05 ）. Bioinformatic analysis revealed that porcine LPL gene encode a protein of 479 amino acids, and its theoretical isoelectric point was 4.99. LPL protein was a hydrophobic and secreted protein with a signal pep- tide. In addition, Suzhong pig was closed to cattle, sheep and cat in phylogenetic tree. These findings suggest that the LPL may play an important role in regulating fat deposition in tissues of Suzhong pigs, such as subcutaneous fat, heart, kidney and muscle, etc, and it can be an impor-tant candidate gene for improving meat quality in Suzhong pigs.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2012年第12期1871-1878,共8页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家生猪现代产业技术体系南京综合试验站(nycytx-009)
国家自然科学基金(31201767)
江苏省博士后科研资助计划经费
江苏省农业科学院博士后基金(026056511104)
江苏省农业科技自主创新基金项目(CX(10)121)
