摘要
Lipoprotein lipase (LPL) gene is a multifunctional protein, playing a major role in the hydrolysis of triglycerides in chylomicrons and very low density lipoproteins (VLDL). According to cDNA of Landrace breed (GenBank accession: X62984), a pair of primers was designed for amplification of intron 3. Sequencing analysis indicated that a G1200A exits in Large White breed by cloning and sequencing. The mutation can be detected by PCR-EcoT22I-RFLP. Polymorphism analysis in a resource family showed that significant difference exits in carcass traits. Pigs with AA genotype had more 6th-7th rib fat thickness (13%,P< 0.05), thorax-waist-fat thickness (11.7%,P=0.065 3), buttock fat thickness (13.1%,P=0.073 0) and average fat thickness (10.4%,P=0.050 3) than pigs with BB genotype. LPL gene showed mainly in the pattern of additive effect and all the dominant effect were not significant. The value of additive effect of 6th-7th rib fat thickness, buttock fat thickness and average fat thickness was -0.17±0.07(P< 0.05),-0.12±0.06( P< 0.05),-0.12±0.06(P< 0.05),respectively. The allelic frequency is significantly different between indigenous Chinese breeds and European breeds.
Lipoprotein lipase (LPL) gene is a multifunctional protein, playing a major role in the hydrolysis of triglycerides in chylomicrons and very low density lipoproteins (VLDL). According to cDNA of Landrace breed (GenBank accession: X62984), a pair of primers was designed for amplification of intron 3. Sequencing analysis indicated that a G1200A exits in Large White breed by cloning and sequencing. The mutation can be detected by PCR-EcoT22I-RFLP. Polymorphism analysis in a resource family showed that significant difference exits in carcass traits. Pigs with AA genotype had more 6th-7th rib fat thickness (13%,P< 0.05), thorax-waist-fat thickness (11.7%,P=0.065 3), buttock fat thickness (13.1%,P=0.073 0) and average fat thickness (10.4%,P=0.050 3) than pigs with BB genotype. LPL gene showed mainly in the pattern of additive effect and all the dominant effect were not significant. The value of additive effect of 6th-7th rib fat thickness, buttock fat thickness and average fat thickness was -0.17±0.07(P< 0.05),-0.12±0.06( P< 0.05),-0.12±0.06(P< 0.05),respectively. The allelic frequency is significantly different between indigenous Chinese breeds and European breeds.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2005年第6期627-630,共4页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家"973"(G2000016105)
"863"(2001AA243031)
博士点基金(20020504006)
