摘要
目的:观察叶酸对胚胎大鼠心肌细胞Cx43表达的影响,探讨叶酸预防先天性心脏畸形作用的可能机制。方法:受孕大鼠随机分为两组,实验组给予叶酸(2mg/kg/d)灌胃,对照组灌服等量生理盐水。选择ED13.5和ED16.5的胚胎大鼠作为研究对象,SP免疫组织化学染色法观察Cx43在心肌细胞的表达。结果:免疫组织化学染色显示各组胎鼠心肌细胞均可见Cx43阳性表达,阳性产物以颗粒形式散在分布于细胞质内,或呈短线状、链状排列在细胞侧-侧连接处。实验组胎鼠心肌Cx43阳性细胞数明显高于同胎龄对照组(P<0.01)。结论:叶酸可增强胎鼠心肌细胞Cx43的阳性表达。
Objective：To observe the effects of folic acid on expression of Cx43 in myocardial cells of embryonic rat,and investigate the possible mechanisms of folic acid preventing congenital heart disease.Methods：Pregnant rats were randomly divided into 2 groups： the rats in experimental group were lavaged with folic acid（2mg/kg/d）,while rats in control group were lavaged with equivalent physiological saline.SP immunohistochemical staining was used to observe the expression of Cx43 in myocardial cells of ED13.5 and ED16.5 embryonic rats.Results：The Cx43 immunopositive products distributed in cytoplasm of myocardial cells,or located at cell side-side joint in spot-band or chain-shaped.The Cx43 positive myocardial cell number of embryonic rats in experimental group were obviously higher than that of embryonic rats in control group at the same gestational age（P0.01）.Conclusions：Folic acid can increase positive expression of Cx43 in myocardial cells of embryonic rats.
出处
《承德医学院学报》
2012年第4期352-354,共3页
Journal of Chengde Medical University
关键词
叶酸
胎鼠
心肌细胞
CX43
Folic acid
Embryonic rat
Myocardial cell
Cx43
