摘要
目的：探讨大鼠心肌细胞间隙连接蛋白43(Cx43)表达的发育特性。方法：应用SP免疫组化和图像分析方法,观察胎大鼠、出生1 d、5 d、10 d和25 d大鼠心肌细胞Cx43的蛋白表达。结果：(1)胎鼠心肌细胞Cx43呈斑点状遍布于心肌细胞侧—侧连接、细胞质内和闰盘处。(2)出生后1 d大鼠心肌细胞的Cx43分布与胎鼠相似,5 d和10 d组逐渐向闰盘处聚集。25 d时部分已聚集于端闰盘处,其余仍呈斑点状散在分布。(3)出生前后心室肌细胞Cx43分布有差异。分布密度总规律是：胎大鼠＞出生1 d＞5 d＞10 d＞25 d。结论：出生前后大鼠心肌细胞Cx43分布随心脏发育逐渐向闰盘处重排,其分布密度呈逐渐下降趋势。
Objective： To investigate the developmental characteristics of connexin 43 （Cx43） expression in rat myocardium. Methods： The expression of Cx43 in fetus and in rats of postnatal 1 d, 5 d, 10 d, 25 d was determined by immunohistochemistry and image analysis. Results ： （1） The Cx43 immunolabelling of fetus rat myocardium had a punctate distribution in cytoplasm and over the entire surface of the myocardium, and few located at intercalated disk. （2） The expression of Cx43 in postnatal rat myocardium was similar to that in fetus myocardiurn. On the 25th day, the granule of Cx43 gathered gradually to the intercalated disk, most of which gathered within the transversely orientated intercalated disk besides and few distributed over the entire surface of rat myocardium. （3） The distributed density of Cx43 of ventricular myocytes in fetus was different from that of the postnatal （fetus〉postnatal 1 d〉5 d〉10 d〉25 d）. Conclusion： The Cx43 is confined to the site of the intercalated disk during heart development. The distributed density of Cx43 decreases gradually.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2006年第6期702-704,共3页
Chinese Journal of Anatomy
基金
河南省自然科学基金(20013100004)
