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ABCG2慢病毒过表达载体构建及其转染人乳腺癌细胞微球体细胞

Construction of recombinant lentiviral over expression vector carrying ABCG2 and its effect on human breast mammospheres-derived cells
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摘要 目的:构建ABCG2过表达慢病毒载体,并在人乳腺癌细胞微球体细胞(mammospheres-derived cells,MSDCs)中建立稳定表达模型。方法:应用ABCG2过表达慢病毒载体转染由无血清悬浮培养的MSDCs,并计算其转染效率。结果:成功构建ABCG2过表达慢病毒载体,在MSDCs中其转染效率为92.4±1.8%。结论:ABCG2过表达慢病毒载体能在MSDCs中稳定表达。 Objective： To construct a lentiviral overexptession vector carrying ABCG2, and establish a stable experession model of mammospheres - derived cells （MSDCs）. Methods： The lentiviral over expression vector carrying ABCG2 was transduced into MSDCs, and the transduction efficiency was calculated. Results：The lentiviral over expression vector carrying ABCG2 was constructed suecesdully, and the transduction efficiency was 92.4 ± 1.8 %. Canclusion：The lentiviral over expression vector carrying ABCG2 can be expressed stably in MSDCs.
作者 范原铭 吴诚义 屈洪波
机构地区 重庆医科大学附属第一医院内分泌乳腺外科
出处 《激光杂志》 CAS CSCD 北大核心 2012年第6期77-78,80,共3页 Laser Journal
关键词 慢病毒 ABCG2 肿瘤干细胞 lentivi.rus ABCG2 tumor stem cells
分类号 R477 [医药卫生—护理学]
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参考文献7

  • 1Huls M, Russel FGM, et al. The Role of ATP Binding Cassete Transporters in Tissue Defense and Organ Regeneration[ J).Journal of Phangaeology and .Experimental Therapeutics, 2009,328(1) :3 - 9-.
  • 2董华英,吴诚义,陈元文.乳腺癌新辅助化疗后的癌干细胞分离培养[J].第三军医大学学报,2010,32(20):2172-2176. 被引量：11
  • 3Sakata S, Fujiwara M, Ohtsuka K, et al. ATP - binding cassette transporters in primary central nervous system lymphoma: Decreased expression of MDR1 P - glycoprotein and breast cancer resistance protein in tumor capillary endothelial cells [ J ]. Oncol Rep, 2011, 25 (2) :333 - 9.
  • 4Schwabedissen HE, Kroemer HK. In Vitro and In Vivo Evidence for the Importance ff Breast Cancer Resistance Protein Transporters (BCRP/MXR/ABCP/ABCG2) [J] . Handb Exp Pharmacol, 2011, 201:325 - 71.
  • 5Krishnamurthy P, Schuetz JD. Role of ABCG2/BCRP in Biology[J]. Annu Rev l:aammeol Toxieol,2006,46:381-410.
  • 6Doyle LA, Ross DD. Multidrug resistance mediated by the breast cancer resistance protein BCRP ( ABCG2 ) [J]. Oncogene, 2007, 22 (47) :7340 - 7358.
  • 7Li WQ, Li YM, Tao BB. Downregulation ff ABCG2 expression in glioblastoma cancer stem cells with miRNA - 328 may decrease their chemoresistance[J]. Med Sci Monit,2010,16(10) : 27 - 30.

二级参考文献21

  • 1Phillips T M, McBride W H, Pajonk F. The response of CD24 (-/low)/CD44 + breast cancer-initiating cells to radiation[J].J Natl Cancer Inst, 2006, 98(24) : 1777 -1785.
  • 2Li X, Lewis M T, Huang J, et al. Intrinsic resistance of tumorigenic breast cancer cells to chemotherapy [J]. J Natl Cancer Inst, 2008, 100(9) : 672 -679.
  • 3Yu F, Yao H, Zhu P, et al. let-7 regulates self renewal and tumorigenicity of breast cancer cells[J]. Cell, 2007, 131 (6) : 1109 - 1123.
  • 4Li H Z, Yi T B, Wu Z Y. Suspension culture combined with chemotherapeutic agents for sorting of breast cancer stem cells [ J]. BMC Cancer, 2008, 8: 135.
  • 5Dey D, Saxena M, Paranjape A N, et al. Phenotypic and functional characterization of human mammary stem/progenitor cells in long term culture[ J] . PLoS One, 2009, 4(4) : e5329.
  • 6Ponti D, Costa A, Zaffaroni N, et al. Isolation and in vitro propagation of tumorigenic breast cancer cells with stem/progenitor cell properties [J]. Cancer Res, 2005, 65(13):5506-5511.
  • 7Grimshaw M J, Cooper L, Papazisis K, et al. Marnmosphere culture of metastatic breast cancer cells enriches for tumorigenic breast cancer cells[J]. Breast Cancer Res, 2008, 10(3) : R52.
  • 8Zhang M, Behbod F, Atkinson R L, et al. Identification of tumor-initiating cells in a p53-null mouse model of breast cancer[J]. Cancer Res, 2008, 68(12): 4674-4682.
  • 9Kok M, Koornstra R H, Margafido T C, et al. Mammosphere-derived gene set predicts outcome in patients with ER-positive breast cancer [J]. JPathol, 2009, 218(3): 316 -326.
  • 10Zhou J, Zhang H, Gu P, et al. Cancer stem/progenitor cell active compound 8-quinolinol in combination with paclitaxel achieves an improved cure of breast cancer in the mouse model[ J]. Breast Cancer Res Treat, 2009, 115 (2) : 269 - 277.

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激光杂志
2012年 第6期

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