摘要
目的:对大鼠灌胃川芎提取物后的吸收入脑成分进行分析鉴定。方法:采用UPLC-Q-TOF-MS对给药脑组织和空白脑组织进行分析,分析总离子流图寻找差异峰,然后根据保留时间、精确分子量、一级和二级质谱数据等鉴定差异峰。结果:川芎提取物给药后,发现3个化合物能透过血脑屏障进入脑组织,经鉴定为洋川芎内酯I,A和藁本内酯。结论:本研究将有助于川芎药效物质基础的阐明。
Objective： To analyze and identify the chemical constituents in rat brain tissues after oral administration of Chuanxlong Rhizoma extracts. Method： The dosed and blank rat brain tissues were analyzed by UPLC-Q-TOF-MS. Different peaks were observed in total ion chromatograms and then identified according to their retention time, accurate mass weight, MS and MS/MS data. Result： After oral administration of Chuanxiong Rhizoma extracts, 3 compounds were absorbed into rat brain tissues through BBB. They were identified as senkyunolide I, senkyunolide A and ligustilide. Conclusion： The study is helpful for interpreting effective sub- stance of Ligusticum chuanxiong.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2012年第23期3647-3650,共4页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(30973873)
关键词
川芎
脑组织
化学成分
鉴定
Ligusticum chuanxiong
brain tissue
chemical constituent
identification
