摘要
目的 观察HSP70反义寡脱氧核苷酸对宫颈癌细胞和卵巢癌细胞热敏感性的影响。方法计数集落形成率 ,了解宫颈癌细胞和卵巢癌细胞的热敏感性。用光镜、琼脂糖凝胶电泳及流式细胞术观察凋亡细胞。结果 经HSP70反义寡脱氧核苷酸预先处理的细胞增强了对热处理的敏感性 ,热敏感性的增加与HSP70反义寡脱氧核苷酸的处理剂量相关。用形态学、琼脂糖凝胶电泳及流式细胞术均证实经HSP70反义寡脱氧核苷酸及热处理的细胞发生了凋亡。凋亡的发生率与HSP70反义寡脱氧核苷酸的处理剂量相关。结论 HSP70反义寡脱氧核苷酸能增强宫颈癌细胞和卵巢癌细胞的热敏感性 ,增强这种热敏感性的可能机理是协同诱导细胞凋亡。
Objective To investigate the thermosensitizing effect of HSP70 antisense oligodeoxynucleolides (ODN) on cervical and ovarian cancer cells.Methods Cervical cancer cell line Hela and ovarian cancer cell line MCAS were used to study their sensitivity to heat (42℃, 1 hr) treatment. Heat induced changes in cell viability was assessed by colony formation assay and apoptosis by cell morphology, agarose gel electrophoresis and flow cytometry.Results Thermosensitivity of Hela and MCAS cells pretreated with HSP70 antisense ODN was increased as compared to cells treated with heat alone. The increase was antisense dose dependent. Although heat treatment or antisense ODN did induce apoptosis of HeLa and MCAS cells, the percentage of apoptotic cells was significantly increased when both heat and antisense ODN were applied. The effect was dependent on the dose of HSP70 antisense ODN.Conclusion The thermosensitivity of ovarian cancer cells and cervial cancer cells can be increased by HSP70 antisense oligomer. Apoptosis may be involved in the increase in thermosensitivity. Subject
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2000年第2期99-101,共3页
Chinese Journal of Oncology
基金
国家杰出青年科研基金!(3972 50 31 )
国家自然科学基金!(39680 0 30及 39740 0 0 6)
四川省卫生厅科研基金
关键词
宫颈癌
卵巢癌
HSP70
反义DNA
温热疗法
Cervical neoplasms
Ovarian neoplasms
Antisense oligodeoxynucleotides
Thermosensitivity
