摘要
目的 观察参附注射液对脊髓缺血性损伤的保护作用 .方法 18只雄性新西兰大白兔 ,随机分为 3组 :A组 (n=6 )即保护组 ,B组 (n=6 )即治疗组 ,C组 (n=6 )即对照组 .A组和 B组分别于缺血前 30 min内或再灌注 30 min内持续恒速静脉输入参附注射液 10 m L· kg- 1 ;采用肾下主动脉阻断法造成脊髓缺血 (2 0 min) ;术后观察神经功能变化并记录 1,4,8,12 ,2 4和 48h神经功能评分 ,48h处死动物后取脊髓(L5～ 7)标本行病理学观察 .结果 术后神经功能评分各时间点 A组和 B组均明显多于 C组 (P<0 .0 5 ) ,A组和 B组间则无差异 ;再灌注 48h脊髓前角正常运动神经元计数 A组和 B组均高于 C组 (P<0 .0 5 ) ,A组和 B组间无差异 .
AIM To investigate the effects of Shenfu, an intravenous drug made from traditional Chinese medicinal herbs, on ischemic spinal cord injury in rabbits. METHODS 18 male New Zealand white rabbits were anesthetized with halothane and spinal cord ischemia was induced for 20 min by infrarenal aortic occlusion. Animals were randomly allo cated to 3 groups: group A ( n =6) and group B ( n =6) received intravenous Shenfu infusion (10 mL·kg -1 ) at a constant rate within 30 min before aortic cross clamping or after reperfusion respectively; group C ( n =6) received no pharmacologic intervention. Neurologic status was scored by the Tarlov system at 1, 4, 8, 12, 24 h and 48 h after the operation. The animals were sacrificed at 48h after reperfusion and the spinal cord (L5~7) was removed immediately for histopathologic study. RESULTS All animals survived the experiment. The neurologic deficit scores at each observation time of group A and group B were significantly higher than those of group C ( P <0.05). No difference was found between group A and B. The numbers of normal neurons at anterior spinal cord of group A and group B were more than those of group C at 48 h after reperfusion ( P <0.05). CONCLUSION Shenfu, either administered before or after aortic occlusion, significantly reduces neurological injury induced by spinal cord ischemia and reperfusion.
出处
《第四军医大学学报》
2000年第3期278-282,共5页
Journal of the Fourth Military Medical University
基金
国家自然科学基金资助项目!(39670699)
关键词
参附注射液
脊髓缺血
再灌注损伤
Shenfu injection
spinal cord ischemia
ischemic and reperfusion injury
