摘要
目的 :研究异丙酚和氯胺酮在脊髓缺血损伤中的保护作用。方法 :2 6只新西兰大白兔随机分为 3组 ,C组为清醒缺血再灌注对照组 ,K组为氯胺酮组 ,P组为异丙酚组。C组与 K组分别于肾下腹主动脉环扎阻断血供前 10分钟给生理盐水 3 ml或氯胺酮 40 m g/kg单次静注 ,P组于同一时间点给 1mg· kg- 1 · m in- 1 异丙酚持续静注直至环扎开放。缺血模型采用肾下腹主动脉环扎模型 ,血液阻断时间 2 5分钟。于再灌注 4、8、12、2 4、48小时进行神经功能评分 ,于再灌注 48小时取材观察腰段脊髓病理改变。结果 :神经功能转归、病理改变和脊髓前角正常神经元数 ,K组及 P组均明显优于 C组 (P均 <0 .0 5 ) ,P组与 K组比较无明显差异。结论 :异丙酚与氯胺酮均能减轻脊髓缺血所致损伤 。
Objective:To evaluate the effects of propofol and ketamine on ischemic spinal cord injury in rabbits.Methods:Infrarenal circumaortic clamping model was used in this study.26 New Zealand white rabbits were randomly assigned to receive normal saline (ischemiareperfusion control group, n =8),propofol(propofol group, n =9) or ketamine (ketamine group, n =9).Normal saline (3 ml) and ketamine (40 mg/kg) were intravenously injected at a single dose 10 minutes before clamping for control group and ketamine group respectively.Propofol ( 1 mg·kg -1 ·min -1 ) was continuously infused intravenously at the same time and continued until the clamping was released for propofol group.Ischemia lasted for 25 minutes.At 4,8,12,24 and 48 hours after reperfusion,neurologic assessment was performed.After the assessment at 48 hours,the rabbits were killed and the spinal cord removed immediately for histopathologic study.Results:The neurologic outcome and pathological change of animals in propofol group and ketamine group were better than those of control group (all P <0 05).The numbers of normal neurons in the anterior spinal cord of propofol group and ketamine group were more than that of control group ( P <0 05).There was no difference between ketamine group and propofol group.Conclusions:Both propofol and ketamine can attenuate the ischemic spinal cord injury in rabbits.
出处
《中国危重病急救医学》
CAS
CSCD
2000年第5期261-263,共3页
Chinese Critical Care Medicine
基金
国家自然科学基金资助项目!(39670699)
