摘要
探讨常氧与缺氧状态下脑啡肽对兔肺动脉平滑肌增殖的调控作用及机制 ,为解决经皮腔内冠脉成形术 (PTCA)支架后冠脉再狭窄问题提供新的理论依据。无菌取出新西兰白兔肺动脉段 ,在常氧和缺氧条件下按照 Ross贴片法培养平滑肌 ,采用相差显微镜、透射电镜和 (-肌动蛋白抗体染色法鉴定平滑肌细胞 ,四唑盐比色法及 3H- Td R参入实验检测平滑肌的生长状况。单纯缺氧明显促进平滑肌细胞的增殖 ;常氧和缺氧状态下脑啡肽显著抑制平滑肌的增生 ;吗啡对平滑肌的生长无明显影响 ;纳洛酮能拮抗常氧和缺氧状态下脑啡肽抑制平滑肌的增殖调控作用。 PTCA支架后冠脉再狭窄的发生可能与
To investigate the effect of endogenous opioid peptide and morphine on proliferation of pulmonary arterial smooth muscle cells under normoxic or hypoxic condition and analyze their mechanism, to provide the theoretical foundation for treating the restenosis of coronary artery after PTCA and stent. Rabbit pulmonary arterial smooth muscle cells are cultured in vitro, MTT method and 3H TdR incorporation were used. The proliferation of pulmonary arterial smooth muscle cells was markedly accelerated under hypoxia condition. L enkephaline significantly inhibited the proliferation of pulmonary arterial smooth muscle cells under normoxic or hypoxic condition, which could be reversed by naloxone. Morphine seemed to have no obvious effect on the proliferation and the DNA synthesis of pulmonary arterial smooth muscle cells. The restenosis of coronary artery after PTCA and stent was related to the depression of plasma L enkephaline level and the hypoxia of newly formed intima of the coronary artery.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2000年第1期37-40,共4页
Medical Journal of Chinese People's Liberation Army
关键词
冠脉再狭窄
缺氧
脑啡肽
冠心病
吗啡
SMC
coronary restenosis
proliferation of arterial smooth muscle cells
hypoxia
L enkephaline
naloxone
