摘要
离体孵育的兔耳动脉条受电场刺激后(强度80v,波宽0.5ms,频率8Hz,20min),其亮氨酸脑啡肽(L-ENK)的含量(pg/mg组织湿重)从正常对照值的38.99±17.29降至12.55±1.96(n=12),甲硫氨酸脑啡肽(M-ENK)的含量从正常对照值的134.67±8.11降至73.68±17.96(n=6),下降均十分显著(P<0.001)。用育亨宾阻断α_2受体后,此效应不受影响。动脉条用阿片受体阻断剂纳洛酮或链霉蛋白酶处理后,电场刺激引起的动脉条收缩反应显著增大,但胰蛋白酶无影响。结果表明,耳动脉内源性ENK可因受电场刺激而释放,α_2受体不参与此释放过程;在2～8Hz的实验范围内,随刺激频率的增加ENK释放可能增加。
After 20 min of electric field stimulation (80 v, 0.5 ms, 8 Hz),L-enkephalin ( L-FNK) content (pg/mg of wet tissue) in the ear arterial strip declined from the control value of 38.99±17.29 to 1155±1.96 (n = 12); M-enkephalin ( M- FNK) from 134.67±8.11 to 73.68±17.96 (n=6), both being significantly decreased (P<0.001). This effect was not influenced after blocking of α2 receptors by yohimbinc. Contractive responses of arterial strips evoked by electric field stimulation were distinctly enhanced after treatment of arte- rial strip with naloxonc, an opioid receptor blocker, and pronase, but unchanged after treatment with trypsin. The results indicate that the release of endogenous FNK can be induced by electric field stimulation, while α2 receptors do not participate in the release course. The amount of released FNK from arterial strips may be increased with the increasing frequencies of stimulation within the range of 2 Hz to 8 Hz.
出处
《第四军医大学学报》
1990年第4期241-244,共4页
Journal of the Fourth Military Medical University
关键词
脑啡肽
放射免疫测定
电场刺激
enkephalins
radioimmunoassay
artery strip
electric field stimulation
