摘要
以常见的7株食用真菌菌株为材料,分别为平菇韩黑(Pleurotus ostreatus hanhei)和平菇8号(P.ostreatus 8)、秀珍菇(P.cornucopiae)、姬菇206(Hypsizygus marmoreus 206)、白灵菇(Pleurotus nebrodensis)、金针菇天福(Flammulina velutipestianfu)和金针菇8802(F.velutipes 8802),筛选出在纤维素平板上生长较快的4株菌(姬菇206、平菇韩黑、平菇8号和秀珍菇),并进一步测定其产生纤维素酶、半纤维素酶和木质素酶的活力以及降解稻草中木质素、纤维素和半纤维素的能力。结果显示:姬菇206中与木质素降解有关的木质素过氧化物酶(LiPs)、锰依赖过氧化物酶(MnPs)和漆酶(Lacs)的活力最大,分别达到58.52、186.66和8.82 U·g-1;滤纸酶活力也是姬菇206最高,为3.86 U·mL-1;平菇韩黑的半纤维素酶活力最高,达到41.20 U·mL-1。稻草降解试验表明,平菇韩黑的半纤维素降解率最高,为51.03%,其次为姬菇206,降解率为50.49%。姬菇206对木质素的分解率最高,达到51.47%。平菇韩黑对稻草的的总降解率最高,达到47.52%。结论:平菇韩黑和姬菇206具有较强的稻草降解能力,可作为降解稻草的工程菌株。
In this study,seven edible fungi were chosen for detecting their abilities of lignin,cellulose and hemicellulose degradation.Four edible fungi which were Pleurotus ostreatus hanhei,P.ostreatus 8,P.cornucopiae,and Hypsizygus marmoreus 206 grew faster on the media with cellulose as the sole carbon source than others.The relative enzyme profiles of the four fungi were examined.The results showed that the fungal H.marmoreus 206 had maximum activities of lignin degrading enzymes,and the values were 58.52 U·g-1,186.66 U·g-1 and 8.82 U·g-1,respectively,for LiPs,MnPs and Lacs.H.marmoreus 206 also produced the maximal filter paper enzyme activity,3.86 U·mL-1.The highest activity of xylanase was 41.20 U·mL-1 on the 16 day by P.ostreatus hanhei.During the 18 days fermentation,P.ostreatus hanhei had the highest hemicellulose degradation rate（51.03%）,and the second was H.marmoreus 206（50.49%）.While H.marmoreus 206 had the highest lignin degradation rate（51.47%）.All in all,P.ostreatus hanhei displayed the maximum rice straw degradation rate（47.52%）,compared to that of H.marmoreus 206（41.80%）,P.ostreatus 8（36.00%）,P.cornucopiae（35.63%）.It could be concluded that P.ostreatus hanhei and H.marmoreus 206 showed the higher straw degradation abilities and could be regarded as potential straw degradation fungi.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2012年第6期49-54,共6页
Journal of Nanjing Agricultural University
基金
国家973计划项目(2011CB100503)
国家公益性行业(农业)科研专项(201103004)
关键词
筛选
食用真菌
木质纤维素
降解
screening
edible fungi
lignocellulolse
degradation
