摘要
研究了白腐菌及纤维素复合酶对稻草秸秆的协同生物降解。结果表明,利用黄孢原毛平革菌固态发酵稻草秸秆的过程中,LiP和MnP的最大活力可以达到28.3U/g和12.6U/g,同时,秸秆中的木质素能被有效降解,但纤维素、半纤维素降解率较低。添加黑曲霉所产的纤维素复合酶能有效地促进秸秆腐熟程度。在接入白腐菌培养10d后,每克稻草添加3IU纤维素酶液并酶解48h可以使稻草秸秆中纤维素降解53.8%,半纤维素降解57.8%,木质素降解44.5%,干物质损失46.3%。此时细胞壁出现大范围破损,整个组织变得松散,秸秆完全腐熟。
Rice straw degradation with white rot fungi and cellulose multienzyme was studied. The results indicated that the LiP, MnP activity produced by P. chrysosoporium 172 could reach 28.3U/g and 12.6U/g respectively under suitable culture condition. And the lignin was degraded efficiently in contrast with the cellulose and hemicellulose in rice straw solid fermentation. Following treatment with white rot fungi, using multienzyme produced by A. niger NL-1 could greatly accelerate the decomposition of rice straw. The decomposition rate of cellulose, hemicellulose, lignin and the loss rate of dry material were 53.8% ,57.8% ,44.5% and 46.3% respectivity when hydrolysis rice straw 48h with cellulase muhienzyme （including 3 IU /g rice straw） after culture P. chrysosoporium 172 for 10 days. Scanning electron microscope analysis showed the cell wall of rice straw was destroyed severely, and the whole tissue got loosely. These results demonstrated the rice straw had been decomposed efticiently and completely.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2007年第3期71-75,共5页
China Biotechnology
基金
国家教育部科学技术研究重点项目(204054)
江苏省教委自然科学研究基金项目(00KJB220002)
南京市科委农业科技项目(200301050)
关键词
生物降解
稻草秸秆
白腐菌
复合酶
Biodegradation
Rice straw
White rot fungi
Muhienzyme
