摘要
从宁波洋沙山海域采集样品,通过刚果红染色法共筛选到23株具有纤维素分解能力的菌株。根据复筛结果,选择其中一株高产碱性纤维素酶的海洋细菌MB117进行深入研究。经16S rDNA鉴定,MB117为交替假单胞菌属(Pseudoalteromonas sp.)细菌。发酵条件研究结果表明,MB117菌株培养的最适pH为7.0,1%接种量和10%装液量最利于产酶,低温有助于菌体生长和产酶。酶学性质研究结果表明,酶反应的最适pH为9.0,pH 7.0～10.0范围内酶活力均可保持在85%以上;MB117菌株CMCase不耐高温,酶最适反应温度为40℃。
A total of 23 cellulase-producing marine bacteria had been isolated from Ningbo Sea area by the CMC/Congo Red method.Then the alkaline cellulase-producing strain MB117 had been made an intensive study of its production of cellulase.According to the 16S rDNA analysis,the strain belonged to the Pseudoalteromonas sp..The optimum initial pH of culture medium for the cullulase production was 7.0.And with 1%-inoculum and 10%-broth content,the maximum production of cellulase can be obtained.Low temperature is good for the growth and cullulase-producing of MB117.The optimum pH for cellulase activity was 9.0,and the strain has a high enzyme activity and a good stability in the basic conditions.The optimum temperature for cellulase activity was 40 ℃.
出处
《上海海洋大学学报》
CAS
CSCD
北大核心
2012年第6期1053-1057,共5页
Journal of Shanghai Ocean University
基金
浙江省教育厅科研项目(Y201019046)
宁波市自然科学基金(2011A610028)
关键词
纤维素酶
海洋细菌
菌株筛选
发酵条件
酶学性质
cellulase
marine bacterium
strain screening
fermentation conditions
proterties of cellulase
